• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Hepatology: The Journal of the European Association for the Study of the Liver >Visualization of the transport of primary and secondary bile acids across liver tissue in rats: in vivo study with fluorescent bile acids.
【24h】

Visualization of the transport of primary and secondary bile acids across liver tissue in rats: in vivo study with fluorescent bile acids.

机译：在大鼠肝组织中一级和二级胆汁酸运输的可视化：荧光胆汁酸的体内研究。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND/AIMS: Lysyl fluorescein conjugated bile acid analogues (LFCBAA) closely parallel their natural counterparts. To assess LFCBAA as a tool for the visualization of bile acid transport within liver tissue. METHODS: Wistar rats were administered physiological concentrations of the primary bile acid analogue cholyllysyl fluoroscein (CLF) and of the secondary bile acid analogue lithocholyllysyl fluorescein (LLF) and serial liver biopsies were taken at fixed intervals. Both compounds were also injected retrogradely into the biliary tree. Frozen sections were examined by fluorescence microscopy. RESULTS: Both CLF and LLF were rapidly taken up from sinusoidal blood but differed significantly in their hepatic handling. CLF was rapidly transported into bile, whereas LLF transport was slower and produced significantly more bile duct fluorescence. LLF clearance showed a lobular gradient with last remaining bile acid being confined largely to zone 3. Both compounds were avidly taken up by cholangiocytes after injection intravenously or retrogradely into the biliary tree. CONCLUSIONS: Visualization of LFCBAA by fluorescence microscopy may yield further information regarding hepatobiliary bile acid localization during studies of physiological and pathological mechanisms involved in transport of bile acids. The presence of both compounds within cholangiocytes strongly suggests that they may undergo a degree of chole-hepatic recirculation.
机译：背景/目的：赖氨酰荧光素缀合的胆汁酸类似物（LFCBAA）与其天然对应物非常相似。评估LFCBAA作为可视化肝组织内胆汁酸运输的工具。方法：对Wistar大鼠进行生理学浓度的原发性胆汁酸类似物胆汁赖氨酰荧光素（CLF）和次生胆汁酸类似物胆汁酸赖氨酰荧光素（LLF）的测定，并按固定间隔进行一系列肝活检。两种化合物也被逆行注入胆道树。通过荧光显微镜检查冷冻切片。结果：CLF和LLF均从正弦血中迅速吸收，但肝脏处理方式差异显着。 CLF迅速转运到胆汁中，而LLF转运较慢，并产生更多的胆管荧光。 LLF清除显示小叶梯度，最后剩余的胆汁酸主要限制在3区。两种化合物在静脉内或逆行注入胆汁树后均被胆管细胞强烈吸收。结论：在研究胆汁酸转运的生理和病理机制过程中，通过荧光显微镜观察LFCBAA可能会提供有关肝胆汁胆汁酸定位的进一步信息。胆管细胞中两种化合物的存在强烈表明它们可能会经历一定程度的胆肝循环。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号