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首页> 外文期刊>Journal of Geophysical Research. Biogeosciences >Diversity and distribution of subseafloor Thermococcales populations in diffuse hydrothermal vents at an active deep-sea volcano in the northeast Pacific Ocean
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Diversity and distribution of subseafloor Thermococcales populations in diffuse hydrothermal vents at an active deep-sea volcano in the northeast Pacific Ocean

机译：东北太平洋深海活火山中弥散热液喷口中海底热球藻种群的多样性和分布

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摘要

The presence, diversity, and distribution of a key group of subseafloor archaea, the Thermococcales, was examined in multiple diffuse flow hydrothermal vents at Axial Seamount, an active deep-sea volcano located in the northeast Pacific Ocean. A polymerase chain reaction (PCR) approach was used to determine if this group of subseafloor indicator organisms showed any phylogenetic distribution that may indicate distinct subseafloor communities at vents with different physical and chemical characteristics. Targeted primers for the Thermococcales 16S rRNA (small subunit ribosomal RNA) gene and intergenic transcribed spacer (ITS) region were designed and applied to organisms filtered directly from a variety of diffuse flow vents. Thermococcales were amplified from 9 of 11 samples examined, and it was determined that the ITS region is a better phylogenetic marker than the 16S rRNA in defining consistent groups of closely related sequences. Results show a relationship between environmental clone distribution and source vent chemistry. The most highly diluted vents with elevated iron and alkalinity contained a distinct group of Thermococcales as defined by the ITS region, suggesting separate subseafloor Thermococcales populations at diffuse vents within the Axial caldera.
机译：在位于东北太平洋的深海活火山Axial Seamount的多个漫流热液喷口中，研究了海底古细菌的一个关键群的存在，多样性和分布。聚合酶链反应（PCR）方法用于确定这组海底指示生物是否显示任何系统发育分布，这可能表明在具有不同物理和化学特征的通风口处存在不同的海底群落。设计了嗜热球菌16S rRNA（小亚基核糖体RNA）基因和基因间转录间隔区（ITS）区域的靶向引物，并将其应用于直接从各种扩散流孔过滤的生物。从所检查的11个样品中的9个中扩增了热球菌，并且确定ITS区在定义紧密相关序列的一致组方面比16S rRNA是更好的系统发生标记。结果表明环境克隆分布与源排放化学之间的关系。铁含量和碱度最高的稀释度最高的通风口包含一组由ITS区域定义的嗜热球菌，这表明在轴向破火山口内的散布出口处有单独的海底嗜热球菌种群。

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