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首页> 外文期刊>Journal of Immunological Methods >New HLA-A, -B, and -C locus-specific primers for PCR amplification from cDNA: application in clinical immunology.
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New HLA-A, -B, and -C locus-specific primers for PCR amplification from cDNA: application in clinical immunology.

机译：用于从cDNA进行PCR扩增的新型HLA-A，-B和-C基因座特异性引物：在临床免疫学中的应用。

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摘要

The individual cellular immune response to intracellular antigens is modeled by the highly polymorphic major histocompatibility complex (HLA) class I molecules. The epitopes presented and the T cell repertoire that recognizes them depend on the HLA constitution of the individual. Therefore, to monitor and to modify an individual's HLA class I-driven cellular immune response, it is necessary to know the HLA class I alleles of the person and the possible epitopes of the target antigen presented by those alleles. In particular, this is necessary in order to design peptide-based vaccines and immune therapies for the treatment of diseases caused by viruses, intracellular parasites or cancer, and to monitor the immune response during those treatments. We describe a new set of HLA-A, -B, and -C locus-specific primers for the polymerase chain reaction (PCR) amplification of the whole coding sequence of these genes from complementary DNA (cDNA). We describe their use for typing and for the production of a library of recombinant HLA class I genes. We discuss two downstream applications of this gene collection: production of soluble HLA molecules and discovery of new epitopes.
机译：通过高度多态的主要组织相容性复合体（HLA）I类分子来模拟对细胞内抗原的单个细胞免疫反应。呈现的表位和识别它们的T细胞库取决于个体的HLA组成。因此，为了监测和修饰个人的HLA I类驱动的细胞免疫反应，有必要了解该人的HLA I类等位基因以及这些等位基因呈现的靶抗原的可能表位。特别地，这对于设计用于治疗由病毒，细胞内寄生虫或癌症引起的疾病的基于肽的疫苗和免疫疗法，以及在那些治疗过程中监测免疫应答是必要的。我们描述了一套新的HLA-A，-B和-C基因座特异性引物，用于从互补DNA（cDNA）扩增这些基因的整个编码序列的聚合酶链反应（PCR）。我们描述了它们用于打字和生产重组HLA I类基因文库的用途。我们讨论该基因收集的两个下游应用：可溶性HLA分子的产生和新表位的发现。

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