...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Immunological Methods >Molecular characterization of the circulating anti-HIV-1 gp120-specific B cell repertoire using antibody phage display libraries generated from pre-selected HIV-1 gp120 binding PBLs.
【24h】

Molecular characterization of the circulating anti-HIV-1 gp120-specific B cell repertoire using antibody phage display libraries generated from pre-selected HIV-1 gp120 binding PBLs.

机译：使用从预选的HIV-1 gp120结合PBL生成的抗体噬菌体展示库，对循环的抗HIV-1 gp120特异性B细胞库进行分子表征。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human bone marrow is a major repository for maturated antibody-secreting plasma cells, which produce the majority of the antibodies found in serum, making it an attractive source for generating human immune antibody libraries. Unfortunately, bone marrow is not always readily available and, although human immune libraries can be generated from circulating B cells, the low frequency of antigen-specific B cells in the circulation yield few monoclonal antibodies of interest. We used a pre-selection strategy to enrich for antigen-specific B cells prior to library generation, and applied this approach to evaluate, at a molecular level, the nature of the human anti-HIV-1 gp120 repertoire encoded by circulating B cells. IgG antibody phage display libraries were generated from HIV-1 seropositive individuals using either affinity-selected anti-gp120 IgG-bearing circulating B cells, predominantly exhibiting memory/activated B cell phenotype, or unselected PBMCs. These libraries were selected against HIV-1 gp120, resulting in isolation of a panel of gp120-specific antibodies. Whereas only 2 gp120-specific antibodies were retrieved from the non-pre-selected HIV-1 library, 9 gp120-specific antibodies were retrieved from the 10-fold smaller library generated from the pre-selected B cells, demonstrating the feasibility of the approach. The anti-gp120 antibodies derived from the circulating B cells of HIV-1 donors generally resembles those from bone marrow plasma cells with respect to epitope specificity, affinity and neutralization ability. They exhibit high affinity for gp120, are directed against a variety of epitopes, but rarely exhibit the ability to neutralize HIV-1.
机译：人骨髓是成熟的分泌抗体的浆细胞的主要储存库，浆细胞产生血清中发现的大多数抗体，使其成为生成人免疫抗体库的有吸引力的来源。不幸的是，骨髓并不总是容易获得，尽管人的免疫文库可以从循环的B细胞中产生，但是循环中抗原特异性B细胞的低频率产生的目标单克隆抗体很少。我们使用预选择策略在库生成之前富集了抗原特异性B细胞，并应用这种方法在分子水平上评估了循环B细胞编码的人类抗HIV-1 gp120组成部分的性质。 IgG抗体噬菌体展示文库是由HIV-1血清反应阳性的个体使用亲和力选择的抗gp120 IgG循环B细胞（主要表现出记忆/激活的B细胞表型）或未选择的PBMC产生的。选择这些针对HIV-1 gp120的文库，从而分离出一组gp120特异性抗体。从非预先选择的HIV-1文库中仅检索到2 gp120特异性抗体，而从预先选择的B细胞生成的10倍较小的文库中检索到9 gp120特异性抗体，证明了该方法的可行性。在表位特异性，亲和力和中和能力方面，源自HIV-1供体的循环B细胞的抗gp120抗体通常类似于来自骨髓浆细胞的抗体。它们表现出对gp120的高亲和力，针对多种表位，但很少表现出中和HIV-1的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号