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首页> 外文期刊>Journal of Ichthyology >A Method for the Assessment of Sperm Quality in Fish
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A Method for the Assessment of Sperm Quality in Fish

机译:鱼类精子质量评估方法

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摘要

A method of computer assisted sperm analysis in fish is described. This method is used for assessing the main parameters of sperm motility by means of a microscope and video camera connected to a computer with easily available software. The results obtained during the analysis noticeably depend on the maximum velocity of spermatozoa (the maximum distance covered by a spermatozoon between two subsequent frames of the videotape, which is set in the control window of the plugin "MTrack2" included into the image analyzing software "ImageJ"). The method is illustrated with reference to the analysis of sperm activity in Zebrasoma sco-pas (Acanthuridae), a representative of the ichthyofauna of coral reefs.
机译:描述了一种计算机辅助的鱼类精子分析方法。该方法用于通过连接到计算机的显微镜和摄像机来评估精子运动的主要参数,该摄像机具有易于使用的软件。分析过程中获得的结果明显取决于精子的最大速度(在录像带的两个后续帧之间,精子覆盖的最大距离,这在图像分析软件附带的“ MTrack2”插件的控制窗口中进行设置) ImageJ”)。该方法参考了斑马(斑马鱼)中的精子活性分析进行了说明,斑马鱼是珊瑚礁鱼科鱼类的代表。

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