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首页> 外文期刊>Journal of immunoassay >Monoclonal antibody-based sensitive enzyme-linked immunosorbent assay for murine serum amyloid A.
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Monoclonal antibody-based sensitive enzyme-linked immunosorbent assay for murine serum amyloid A.

机译：基于单克隆抗体的鼠血清淀粉样蛋白A敏感酶联免疫吸附测定

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摘要

Enzyme-linked immunosorbent assay (ELISA) methods for measuring murine serum amyloid A (SAA), a representative acute phase reactant, were developed utilizing a newly produced monoclonal antibody. Two site-ELISA, in which the monoclonal antibody was used as the captured antibody, was sensitive enough to determine the SAA concentration in mice at the steady state. Direct binding ELISA, in which the sample SAA bound to the plastic wells was detected by the antibody, was simple and suitable for measuring the elevated SAA, but could not analyze the resting level of SAA because of the need for high dilution in plasma samples. Plasma SAA concentrations were measured in ten ICR mice on the day of purchase and at the end of seven days of ordinary rearing. The SAA concentration of one animal decreased from 1.6 to 0.5 mg/l during a week, while the others had no obvious changes. The plasma SAA of the ten animals after one week of rearing ranged from 0.3 to 0.8 mg/l with a mean of 0.47. These mice, two days after 10 microg lipopolysaccharide were given, had increased SAA values up to a mean of 300 mg/l, though with variations between animals.
机译：利用新生产的单克隆抗体开发了用于测定鼠类血清淀粉样蛋白A（SAA）（一种代表性的急性期反应物）的酶联免疫吸附法（ELISA）方法。使用单克隆抗体作为捕获抗体的两点酶联免疫吸附测定法足够灵敏，可以确定稳态下小鼠体内的SAA浓度。通过抗体检测与塑料孔结合的样品SAA的直接结合ELISA方法简单易行，适合测量升高的SAA，但由于需要在血浆样品中进行高稀释，因此无法分析SAA的静息水平。在购买当天和正常饲养的七天结束时，在十只ICR小鼠中测量血浆SAA浓度。一只动物的SAA浓度在一周内从1.6毫克/升降低至0.5毫克/升，而其他动物则无明显变化。饲养一周后，十只动物的血浆SAA为0.3-0.8 mg / l，平均值为0.47。这些小鼠在给予10微克脂多糖后两天，其SAA值增加至平均300 mg / l，尽管动物之间存在差异。

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