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首页> 外文期刊>Journal of immunoassay >Development of an immunoassay for the beta-adrenergic agonist ractopamine.
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Development of an immunoassay for the beta-adrenergic agonist ractopamine.

机译：开发β-肾上腺素能激动剂莱克多巴胺的免疫测定法。

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摘要

Antibody generated from ractopamine-hemiglutarate-KLH was used to develop a ractopamine ELISA. The antibody showed good sensitivity in phosphate buffer, with an IC50 of 4.2 ng/ml (ppb) toward ractopamine and 16.2 ng/ml toward glucuronides of ractopamine conjugated to the phenethanolamine phenol of ractopamine. Phenylbutylamine phenol glucuronides of the (RS, SR) ractopamine diastereoisomers showed about 4% cross-reactivity, but the glucuronide of the (RR, SS) diastereoisomers conjugated at the same phenolic group showed no detectable reactivity with the antibody. The antibody generally had cross-reactivity towards compounds with bis-phenylalkyl amine structures rather than compounds with simple branched N-alkyl substituents. For example, the antibody showed little or no cross reactivity towards clenbuterol, isoproterenol, metaproterenol, and salbutamol, but cross-reacted with dobutamine. The system demonstrated a matrix effect similar to other enzyme immunoassays, dilution of urine decreased but did not eliminate the matrix effect.
机译：从莱克多巴胺-半谷氨酸-KLH产生的抗体被用于开发莱克多巴胺ELISA。该抗体在磷酸盐缓冲液中显示出良好的敏感性，对莱克多巴胺的IC50为4.2 ng / ml（ppb），对与莱克多巴胺的苯乙醇胺酚共轭的莱克多巴胺的葡糖醛酸苷的IC50为16.2 ng / ml。 （RS，SR）莱克多巴胺非对映异构体的苯基丁胺酚葡糖醛酸苷显示约4％的交叉反应性，但在相同酚基上共轭的（RR，SS）非对映异构体的葡糖醛酸苷显示与抗体无可检测的反应性。抗体通常对具有双苯基烷基胺结构的化合物具有交叉反应，而不是对具有简单支链N-烷基取代基的化合物具有交叉反应性。例如，该抗体对克仑特罗，异丙肾上腺素，间肾上腺素和沙丁胺醇几乎没有或没有交叉反应性，但与多巴酚丁胺发生了交叉反应。该系统显示出与其他酶免疫测定相似的基质效应，尿液稀释度降低但并未消除基质效应。

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