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首页> 外文期刊>Journal of health science. >Expression of Secretin in Porcine and Rat Central Nervous System
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Expression of Secretin in Porcine and Rat Central Nervous System

机译：分泌素在猪和大鼠中枢神经系统中的表达

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摘要

Many intestinal hormones are also distributed throughout the central nervous system (CNS). However, the existence of secretin, a typical intestinal hormone, has not been clearly shown in the brain. There have been conflicting data concerning the expression of secretin in the CNS. The aim of this study was to confirm the existence of secretin in the CNS immunochemically and histochemically. Proforms of secretin were detected in porcine cortex extracts by gel filtration chromatography and reverse-phase HPLC with a radioimmunoassay using different specific antibodies raised against secretin and prosecretin (1-41), indicating the production and processing of secretin precursor in the CNS. An immunocytochemical search in several regions of the rat CNS with the two antibodies revealed that secretin-positive neurons were concentrated in a limited area ranging from the corner of the dentate gyms to the molecular layer in the hippocampus. In situ hybridization also supported this finding. The limited location of secretin-positive cells shown here caused difficulty in detecting secretin in the CNS. The hippocampus is thought to contribute to aging, and secretinergic neurons in this region might play an important role. This study suggests that secretin is produced in the CNS and may act as a neurotransmitter or neuromodulator in the hippocampus.
机译：许多肠激素也分布在整个中枢神经系统（CNS）中。但是，大脑中尚未清楚显示促胰液素（一种典型的肠道激素）的存在。关于中枢神经系统中secretin表达的数据存在矛盾。这项研究的目的是通过免疫化学和组织化学方法确认中枢神经系统中存在促胰液素。猪皮层提取物中的促胰液素前体通过凝胶过滤色谱和反相HPLC进行放射免疫分析，使用针对促胰液素和促胰泌素原（1-41）的不同特异性抗体进行检测，表明中枢神经系统中促胰液素前体的产生和加工。用两种抗体在大鼠中枢神经系统的几个区域进行的免疫细胞化学检查显示，促胰液素阳性神经元集中在有限的区域内，从齿状健身房的角到海马的分子层不等。原位杂交也支持这一发现。此处显示的促胰液素阳性细胞位置有限，导致在CNS中检测促胰液素困难。海马被认为是导致衰老的原因，该区域的分泌能神经元可能起重要作用。这项研究表明，促胰液素在中枢神经系统中产生，并可能在海马中充当神经递质或神经调节剂。

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