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首页> 外文期刊>Journal of health science. >Isolation and Characterization of a 1,3-Dichloro-2-Propanol-Degrading Bacterium
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Isolation and Characterization of a 1,3-Dichloro-2-Propanol-Degrading Bacterium

机译：1,3-二氯-2-丙醇降解菌的分离与表征

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摘要

Arthrobacter sp. strain PY1, a bacterium having the ability to degrade 1,3-dichlotopropanol (1,3-DCP), was isolated from a soil sample of a chemical plant. Strain PY1 degraded 1000 mg/1 (7.75 mM) of 1,3-DCP completely within 7 days, and the ability was elevated by acclimatization up to 4000 mg/1/week. Addition of nutrients such as peptone, glucose or glycerol showed no or slight effect on the degrading activity. These results suggest that strain PY1 is a useful organism in a biological control system for 1,3-DCP pollution. The ability to degrade 1,3-DCP was induced by addition of 1,3-DCP to the culture of strain PY1. A 1,3-DCP-degrading enzyme (Deh-PYl) was purified from the cytoplasmic fraction of strain PY1 by fractionation with ammonium sulfate, hydrophobic chromatography and anion exchange chromatography. Purified Deh-PYl is a tetramer of a homogeneous subunit having a molecular weight of 20 kDa. Analysis of the N-terminal amino acid sequence of Deh-PYl showed that the 31 residues were quite similar to those of known 1,3-DCP-dehalogenases of other organisms, Arthrobacter sp. strain AD2 and Corynebacterium sp. strain N-1074, although some differences in composition or enzymatic characteristics were observed. The Km value and Vmax of Deh-PYl were 2.67 mM and 7.81 (mu)mol/min/mg, respectively, and the optimum reaction temperature and pH were 40-50(deg)C and 9.5-10.5.
机译：关节杆菌从化工厂的土壤样品中分离出具有降解1，3-二氯异丙醇（1，3-DCP）能力的细菌PY1。 PY1菌株在7天内完全降解了1000 mg / 1（7.75 mM）的1,3-DCP，通过适应性提高能力达到4000 mg / 1 / week。添加蛋白p，葡萄糖或甘油等营养物质对降解活性没有影响或影响很小。这些结果表明，菌株PY1在1,3-DCP污染的生物控制系统中是有用的生物。通过向菌株PY1的培养物中添加1，3-DCP来诱导降解1，3-DCP的能力。通过用硫酸铵分级分离，疏水色谱法和阴离子交换色谱法从菌株PY1的细胞质级分中纯化1，3-DCP降解酶（Deh-PY1）。纯化的Deh-PY1是分子量为20kDa的均质亚基的四聚体。对Deh-PY1的N-末端氨基酸序列的分析表明，这31个残基与其他生物节杆菌属的已知的1，3-DCP-脱卤酶的残基非常相似。菌株AD2和棒杆菌属。菌株N-1074，尽管观察到组成或酶学特性的一些差异。 Deh-PY1的Km值和Vmax分别为2.67mM和7.81μmol/ min / mg，最佳反应温度和pH为40-50℃和9.5-10.5。

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