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首页> 外文期刊>Journal of Insect Physiology >WEB 2086, a platelet-activating factor antagonist, inhibits prophenoloxidase-activating system and hemocyte microaggregation reactions induced by Trypanosoma rangeli infection in Rhodnius prolixus hemolymph
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WEB 2086, a platelet-activating factor antagonist, inhibits prophenoloxidase-activating system and hemocyte microaggregation reactions induced by Trypanosoma rangeli infection in Rhodnius prolixus hemolymph

机译：WEB 2086，一种血小板活化因子拮抗剂，抑制Rhodronius prolixus血淋巴中的锥虫锥虫感染引起的前酚氧化酶活化系统和血细胞微聚集反应

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摘要

The effects of the triazolodiazepine WEB 2086, a platelet-activating factor (PAF) antagonist, on hemocyte microaggregation and prophenoloxidase (proPO)-activating system in the hemolymph, hemocoelic infection and mortality in fifth-instar larvae of Rhodnius prolixus inoculated with Trypanosoma rangeli were investigated. Hemocoelic injection of short T. rangeli epimastigotes (1x10(4) parasites/insect) in R. prolixus that were previously fed with blood containing 1muM of WEB 2086 resulted in (i) reduced hemocyte microaggregations as well as an attenuated proPO system in the hemolymph and (ii) greater parasitemia and mortality among the insects. In vitro assays using hemolymph from insects previously fed with blood containing WEB 2086 exhibited attenuated hemocyte microaggregations when T. rangeli was employed as the inducer of the reaction, and this effect was not counteracted by PAF treatment. In vitro assays using hemolymph from insects previously fed with blood, regardless of WEB 2086 presence increased the PO activity when incubated with the parasites. However, the PO activity was drastically inhibited when hemolymph from insects fed with blood, whether or not it contained WEB 2086, was incubated with fat body homogenates from insects fed with blood containing WEB 2086. The addition of PAF did not enhance the PO activity. These analyses did not reveal any PAF influence on WEB 2086 effects in the two defense reactions.
机译：接种了锥虫的Rhodnius prolixus的五龄幼虫的血小板活化因子（PAF）拮抗剂三唑二氮杂卓WEB 2086对血淋巴细胞微聚集和酚氧化原酶（proPO）活化系统的影响调查。预先在R. prolixus中用短毛T.rangeli附生鞭毛虫（1x10（4）寄生虫/昆虫）进行血流注入，此前曾用含1μMWEB 2086的血液供血，导致（i）血细胞微聚集减少，并且血淋巴中的proPO系统减弱（ii）昆虫中的寄生虫病和死亡率更高。当使用兰氏梭菌作为反应的诱导剂时，使用先前从含有WEB 2086血液的昆虫中获取的昆虫的淋巴进行的体外分析显示出减弱的血细胞微聚集，而PAF处理不能抵消这种影响。不管WEB 2086存在与否，与先前寄生有血的昆虫进行的血淋巴的体外分析均可提高PO活性。但是，当用血液喂养的昆虫（无论是否含有WEB 2086）的血淋巴与来自血液喂养的含有WEB 2086的昆虫的脂肪匀浆一起孵育时，PO活性会受到极大抑制。添加PAF不会增强PO活性。这些分析未显示这两个防御反应中PAF对WEB 2086效应的任何影响。

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