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首页> 外文期刊>Journal of Insect Physiology >Thermal history influences diapause development in the solitary bee Megachile rotundata
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Thermal history influences diapause development in the solitary bee Megachile rotundata

机译：热史影响单蜂Megachile rotundata的滞育发育

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摘要

Respiration rate, time to pupation and the expression patterns of selected genes were examined during the diapause to post-diapause transition in the alfalfa leafcutting bee, Megachile rotundata held at constant 4 degrees C in winter storage. Respiration quotients were at or below 0.7 from December to May and then increased to over 0.8 in June and July. The time required for prepupae to reach the pupal stage following transfer to 29 degrees C decreased from 23 days in December to 10 days in July. HSP70 was expressed at a consistently high level in all the diapausing prepupae stored at 4 degrees C. In contrast, we demonstrated previously that HSP70 expression in diapausing prepupae maintained under field conditions began decreasing after December and was expressed at trace levels in the June samples. Transferring prepupae stored at 4 to 25 degrees C at monthly intervals from December to July induced a significant decrease in HSP70. Levels of HSC70 showed no changes during the transition to post-diapause development in prepupae maintained at 4 degrees C. Transferring the prepupae to 25 degrees C during the April-June time interval elicited an increase in HSC70 expression. HSP90 was expressed at a consistent level in prepupae stored at 4 degrees C but decreased to very low levels after being transferred to 25 degrees C in December-February prepupae: no decrease was noted in the April-July prepupae. Actin was expressed at trace levels in the diapausing prepupae maintained at 4 degrees C and increased slightly in the post-diapausing pupae. Transferring prepupae stored at 4 to 25 degrees C at monthly intervals from December to July induced an increase in actin expression. These results indicate that the level of gene expression for selected genes in diapausing and post-diapause bees is highly influenced by their thermal history.
机译：在苜蓿切叶蜂Megachile rotundata在冬季储存中恒定保持在4摄氏度的滞育至滞育后过渡过程中，检查了呼吸速率，化脓时间和所选基因的表达模式。从12月到5月，呼吸商数等于或低于0.7，然后在6月和7月，呼吸商数增加到0.8以上。转移到29摄氏度后，pre达到ae期所需的时间从12月的23天减少到7月的10天。 HSP70在储存于4摄氏度的所有滞育前足动物中始终以高水平表达。相反，我们先前证明了田间条件下维持的滞育前足动物中的HSP70表达在12月之后开始下降，并在6月样品中以痕量表达。从12月到7月，每月间隔4到25摄氏度存储的足前草转移，导致HSP70显着下降。 HSC70的水平在维持于4摄氏度的足前期向绝经后发展的过程中未显示任何变化。在4月至6月的时间间隔内将足前期转移至25摄氏度会引起HSC70表达的增加。 HSP90在存放于4摄氏度的prepupae中以一致的水平表达，但在12月至2月的prepupae中转移至25°C后降低至非常低的水平：4月至7月的prepupae中没有发现降低。肌动蛋白在维持在4摄氏度的滞育前p中以痕量水平表达，在停滞后的up中略有增加。从12月到7月，每月间隔4到25摄氏度存储的足前草转移，导致肌动蛋白表达增加。这些结果表明，滞育和滞育后蜜蜂中选定基因的基因表达水平受其热史的影响很大。

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