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首页> 外文期刊>Journal of industrial microbiology & biotechnology >Studies on inhibition of transformation of 2,4,6-trinitrotoluene catalyzed by Fe-only hydrogenase from Clostridium acetobutylicum
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Studies on inhibition of transformation of 2,4,6-trinitrotoluene catalyzed by Fe-only hydrogenase from Clostridium acetobutylicum

机译：丙酮丁醇梭菌仅铁加氢酶抑制2,4,6-三硝基甲苯转化的研究

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摘要

The major enzyme in Clostridium acetobutylicum ATCC 824 leading to transformation of TNT has been reported to be the Fe-only hydrogenase. In this study, we examine the effect of inhibitors of hydrogenase on TNT reduction by Clostridial extracts. These experiments further demonstrate the major role of hydrogenase in TNT transformation. The C. acetobutylicum hydrogenase is closely related to that of C. pasteurianum; and can be fitted to the X-ray crystal structure with a root mean square deviation of 1.18 angstroms for the Calpha atoms of the generated 3D simulation model. The Hyd1, Hyd2, and Hyd3 antibodies generated against hydrogenase reacted with both the hydrogenase in cell extracts and with C. acetobutylicum hydrogenase expressed in Escherichia coli. Inhibition studies using antibodies against Fe-only hydrogenase from C. acetobutylicum indicated that the transformation of TNT by crude cell extracts was completely inhibited by Hyd2 antibody (to amino acid 415-428) whereas antibodies Hyd1 (to residues 1-16) and Hyd3 (to amino acid 424-448) inhibited less effectively. The TNT transforming activity of the cell extract was retained when Hyd2 antibody pretreated with purified but enzymatically inactive recombinant hydrogenase was added to the extract. Addition of the transition metal Cu2+ to extracts completely inhibited the transformation of TNT suggesting the destruction of [4Fe-4S] centers which are essential for transfer of electrons from the H2-activating site to TNT. Growth of C. acetobutylicum was also inhibited by 0.5 mM Cu2+ and Hg2+ ions. The triazine dye, procion red and the nitroimidazole drug, metronidazole inhibit TNT reduction. The inhibition studies using antibodies, procion red, metronidazole, and transition metals suggest that different portions of hydrogenase are required for effective TNT reduction.
机译：丙酮丁醇梭菌ATCC 824中导致TNT转化的主要酶据报道是仅铁的氢化酶。在这项研究中，我们检查了氢化酶抑制剂对梭菌提取物减少TNT的作用。这些实验进一步证明了氢化酶在TNT转化中的主要作用。乙酰丁酸梭菌氢化酶与巴氏梭菌密切相关。并且可以针对生成的3D模拟模型的Calpha原子以均方根偏差为1.18埃的X射线晶体结构进行拟合。针对氢化酶产生的Hyd1，Hyd2和Hyd3抗体与细胞提取物中的氢化酶和大肠杆菌中表达的丙酮丁醇梭菌氢化酶反应。使用针对来自丙酮丁醇梭菌的仅铁的氢化酶的抗体进行的抑制研究表明，粗细胞提取物对TNT的转化被Hyd2抗体（针对氨基酸415-428）完全抑制，而抗体Hyd1（针对残基1-16）和Hyd3（针对残基1-16）。 （对氨基酸424-448）的抑制作用较差。当将经纯化但无酶活性的重组氢化酶预处理的Hyd2抗体加入提取物中时，细胞提取物的TNT转化活性得以保留。向萃取物中添加过渡金属Cu2 +完全抑制了TNT的转化，表明[4Fe-4S]中心的破坏，这对于电子从H2活化位点到TNT的转移至关重要。丙酮丁醇梭菌的生长也受到0.5 mM Cu2 +和Hg2 +离子的抑制。三嗪染料，procion红和硝基咪唑药物，甲硝唑抑制TNT的降低。使用抗体，蛋白红，甲硝唑和过渡金属进行的抑制研究表明，有效降低TNT所需的氢化酶部分不同。

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