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首页> 外文期刊>Journal of industrial microbiology & biotechnology >Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1
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Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1

机译：产生生物表面活性剂的菌株Brevibacilis brevis HOB1的分离和鉴定

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Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis HOB1. It has been investigated using different carbon and nitrogen sources. It showed that the strain was able to grow and reduce the surface tension of the broth to 29 mN/m on commercial sugar and maltose, and to 32 mN/m on glucose after 72 h of growth. The maximum amount of biosurfactant was obtained when nitrate ions were supplied as nitrogen source. Biosurfactant produced by Brevibacilis brevis HOB1 was confirmed as a lipopeptide class of biosurfactant using TLC test and mass spectra. Lipopeptide isoforms were isolated from cell-free supernatants by acid-precipitation followed by one step of chromatographic separation on solid-phase ODS C18 column. The separation was confirmed by HPLC and ESI Q-TOF MS spectroscopy. Comparing the mass data obtained and the mass numbers reported for the lipopeptide complexes from other strains, it can be concluded that the major lipopeptide product of Brevibacilis brevis HOB1 is the surfactin isoform. This lipopeptide showed strong antibacterial and antifungal activity. It is a candidate for the biocontrol of pathogens in agriculture and other industries.
机译：从油田的生产水中分离出产生生物表面活性剂的细菌。使用表面张力测试筛选分离物的生物表面活性剂生产。表面张力的最大降低是通过细菌菌株实现的，该菌株通过16S rRNA基因测序鉴定为短杆菌Brevibacilis brevis HOB1。已经使用不同的碳和氮源进行了研究。结果表明，该菌株能够生长并在72小时的生长后，将肉汤的表面张力在商品糖和麦芽糖上降至29 mN / m，在葡萄糖上降至32 mN / m。当提供硝酸根离子作为氮源时，可获得最大量的生物表面活性剂。通过TLC测试和质谱，确认了由短杆菌Brevibacilis brevis HOB1产生的生物表面活性剂是脂肽类生物表面活性剂。通过酸沉淀从无细胞上清液中分离脂肽同工型，然后在固相ODS C18色谱柱上进行色谱分离的一步。分离通过HPLC和ESI Q-TOF MS光谱法确认。比较获得的质量数据和报告的其他菌株脂肽复合物的质量数，可以得出结论，短杆菌Brevibacilis brevis HOB1的主要脂肽产物是表面活性素同工型。该脂肽显示出强的抗菌和抗真菌活性。它是农业和其他行业中病原体生物防治的候选者。

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