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首页> 外文期刊>Journal of fruit and ornamental plant research >APPLICATION OF THERMO- AND CHEMOTHERAPY IN VITRO FOR ELIMINATING SOME VIRUSES INFECTING Prunus sp. FRUIT TREES
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APPLICATION OF THERMO- AND CHEMOTHERAPY IN VITRO FOR ELIMINATING SOME VIRUSES INFECTING Prunus sp. FRUIT TREES

机译：热疗法和化学疗法在体外用于消除感染李属李子的某些病毒的应用。水果树

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摘要

Thermotherapy and chemotherapy in vitro were applied to eliminate Apple chlorotic leaf spot virus (ACLSV) and Prunus necrotic ring spot virus (PNRSV) from myrobalan (Prunus cerasifera var. divaricata Borgh.), PNRSV from 'Empress' plum (Prunus domestica L.) and Prune dwarf virus (PDV) from 'Early Rivers' sweet cherry (Cerasus avium (L.) Moench.) plants. ELISA assays conducted one year after therapy indicated that thermotherapy in vitro was highly effective for PNRSV and ACLSV elimination but it was not an efficient method for obtaining PDV-free 'Early Rivers' sweet cherry. Virazole~(R) (Ribavirin) at concentration 25-100 mg l~(-1) was effective in eliminating ACLSV from myrobalan and PNRSV from 'Empress' plum shoots but this therapy was not successful in eliminating PNRSV from myrobalan and PDV from 'Early Rivers' sweet cherry shoots. Combination of thermotherapy with Virazole~(R) treatment at concentration 50-100 mg l~(-1) enabled to eliminate PDV from 'Early Rivers' sweet cherry shoots and PNRSV from majority of treated shoots of myrobalan and 'Empress' plum. The higher concentrations of Virazoie~(R) (50-100 mg l~(-1)) were phytotoxic for treated shoots, especially in case of 'Empress' plum.
机译：应用体外热疗法和化学疗法消除了来自myrobalan（Prunus cerasifera var。divaricata Borgh。）的苹果绿化叶斑病毒（ACLSV）和李属坏死环斑病毒（PNRSV），消除了来自“ Empress”李子（Prunus domestica L.）的PNRSV。和来自'Early Rivers'甜樱桃（Cerasus avium（L.）Moench。）植物的西梅矮病毒（PDV）。治疗后一年进行的ELISA分析表明，体外热疗对消除PNRSV和ACLSV非常有效，但它不是获得不含PDV的“早河”甜樱桃的有效方法。 （Ribavirin）浓度为25-100 mg l〜（-1）可以有效地消除'Empress'李子芽中的ACLSV和PNRSV，但是这种疗法不能成功地消除'Empress'李子中的PNRSV和PDV。早期河流的甜樱桃芽。浓度为50-100 mg l〜（-1）的热疗与Virazole〜的结合，可以消除'早河'甜樱桃芽中的PDV和大部分经处理的myrobalan和'Empress'李子芽中的PNRSV。较高浓度的Virazoie？（50-100 mg l〜（-1））对处理过的芽有植物毒性，特别是在“ Empress”李子的情况下。

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