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首页> 外文期刊>Journal of Inorganic Biochemistry: An Interdisciplinary Journal >Synthesis of biocompatible polymeric nano-capsules based on calcium carbonate: A potential cisplatin delivery system
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Synthesis of biocompatible polymeric nano-capsules based on calcium carbonate: A potential cisplatin delivery system

机译：基于碳酸钙的生物相容性聚合物纳米胶囊的合成：潜在的顺铂递送系统

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A smart nanocarrier system for cancer therapy, based on a recently developed technique for preparing pure nanometric calcium carbonate (CaCO3), was studied. Different approaches were used to obtain sustained release of cisplatin: at first, pure CaCO3 nanoparticles were evaluated as carriers, then the nanoparticles were functionalized with polymer or silanes, and finally they were employed as a substrate to build layer by layer (LbL) self-assembled polyelectrolyte nanocapsules. Loading efficiency and release kinetics were measured. The best loadings were obtained with the LbL nanocapsules, allowing for high loading efficiency and the possibility of controlling the release rate of the drug. The behavior of all the carriers was evaluated on four neoplastic cell lines, representative of different types of neoplastic disease, namely MCF-7 (breast cancer), SKOV-3 (ovarian cancer), HeLa (cervical cancer) and CACO-2 (human epithelial colorectal adenocarcinoma). Negligible cytotoxicity of the nanoparticles, functionalized nanoparticles, and nanocapsules was observed in experiments with all cell lines. Nanocapsules were functionalized with fluorescein isothiocyanate (FTTC) in order to track their kinetic of internalization and localization in the cell line by confocal laser scanning microscopy (CLSM). The cytotoxicity of the loaded capsules was evaluated, showing cell survival rates close to those expected for non-encapsulated cisplatin at the same nominal concentration. (C) 2015 Elsevier Inc. All rights reserved.
机译：研究了一种基于癌症的智能纳米载体系统，该系统基于最近开发的用于制备纯纳米碳酸钙（CaCO3）的技术。使用不同的方法来获得顺铂的持续释放：首先，将纯CaCO3纳米颗粒作为载体进行评估，然后将纳米颗粒用聚合物或硅烷官能化，最后将它们用作底物以自我构建逐层（LbL）组装的聚电解质纳米胶囊。测量加载效率和释放动力学。 LbL纳米胶囊获得了最佳的负载量，从而实现了较高的负载效率并可以控制药物的释放速率。在四种代表不同类型肿瘤疾病的肿瘤细胞系（即MCF-7（乳腺癌），SKOV-3（卵巢癌），HeLa（宫颈癌）和CACO-2（人类））上评估了所有携带者的行为上皮结肠直肠腺癌）。在所有细胞系的实验中，纳米颗粒，功能化的纳米颗粒和纳米胶囊的细胞毒性均可以忽略不计。纳米胶囊用荧光素异硫氰酸酯（FTTC）进行功能化，以便通过共聚焦激光扫描显微镜（CLSM）跟踪其内在化和在细胞系中的定位动力学。评估了加载的胶囊的细胞毒性，显示出在相同的标称浓度下，细胞存活率接近未包封的顺铂所预期的细胞存活率。 （C）2015 Elsevier Inc.保留所有权利。

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