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首页> 外文期刊>Journal of industrial and engineering chemistry >Amperometric cholesterol biosensor using layer-by-layer adsorption technique onto electrospun polyaniline nanofibers
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Amperometric cholesterol biosensor using layer-by-layer adsorption technique onto electrospun polyaniline nanofibers

机译：使用层层吸附技术在电纺聚苯胺纳米纤维上的安培胆固醇生物传感器

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摘要

An amperometric cholesterol biosensor was fabricated using electrospun polyaniline nanofibers. Polyaniline was dissolved in chloroform with camphorsulfonic acid, and polystyrene was added in this solution. Using this mixed solution, nanofibers were formed and collected by electrospinning. Then cholesterol oxidase was immobilized onto these fibers using an electrostatic layer-by-layer adsorption technique. Poly(diallyldimethylammonium chloride) was used as the counter ion source. The level of adsorption was examined and evidence of layer-by-layer adsorption was investigated using a quartz crystal microbalance (QCM) technique. A cholesterol biosensor was fabricated from these nanofibers as a working electrode, and it was used to measure the cholesterol concentration.
机译：使用电纺聚苯胺纳米纤维制造了安培胆固醇生物传感器。用樟脑磺酸将聚苯胺溶解在氯仿中，并在该溶液中加入聚苯乙烯。使用这种混合溶液，形成纳米纤维并通过电纺丝收集。然后使用静电逐层吸附技术将胆固醇氧化酶固定在这些纤维上。聚（二烯丙基二甲基氯化铵）用作抗衡离子源。使用石英晶体微量天平（QCM）技术检查吸附水平并研究逐层吸附的证据。由这些纳米纤维作为工作电极制造了胆固醇生物传感器，并用于测量胆固醇浓度。

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