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首页> 外文期刊>Journal of industrial and engineering chemistry >Use and recycling of Ca-alginate biocatalyst for removal of phenol from wastewater
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Use and recycling of Ca-alginate biocatalyst for removal of phenol from wastewater

机译：海藻酸钙生物催化剂的使用和回收用于去除废水中的苯酚

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摘要

The objective of the current work is the exhaustive study of the phenol degradation potential in both free cell and immobilized bacterium (Pseudomonas aeruginosa) in calcium alginate beads (biocatalyst) was investigated for its ability to grow and degrade phenol as its sole source of carbon and energy. The biodegradation assays were performed in liquid medium with phenol being the only substrate. It was found that P. aeruginosa is able to degrade phenol up to 500 mg L~(-1) in 50 h as free cell and 900 mg L~(-1) in 80 h when immobilized in the calcium alginate beads. However, for 1200 mgL~(-1) concentration, the immobilized cells took much more time (290 h) for a complete degradation. The reuse of these beads in different concentrations of phenol (100-900 mg L~(-1)) showed that the cells keep their phenol degradation ability up to 900 mg L~(-1)in 78.5 h with 99% removal efficiency. Similarly, the reuse of the biocatalyst in the same initial phenol concentration (500 mg L~(-1)), allows us to get 9 cycles.
机译：当前工作的目的是详尽研究藻酸钙珠（生物催化剂）中游离细胞和固定细菌（铜绿假单胞菌）中苯酚的降解潜力，研究其生长和降解苯酚作为其唯一碳源的能力。能源。在液体培养基中进行生物降解测定，其中苯酚是唯一的底物。发现铜绿假单胞菌在固定于藻酸钙珠粒后，能够在50小时内降解为游离细胞的苯酚高达500 mg L〜（-1），在80小时内降解为900 mg L〜（-1）。然而，对于1200 mgL〜（-1）的浓度，固定化细胞要花更多的时间（290小时）才能完全降解。这些珠子在不同浓度的苯酚（100-900 mg L〜（-1））中重复使用表明，细胞在78.5 h内可保持高达900 mg L〜（-1）的苯酚降解能力，去除率达99％。同样，在相同的初始苯酚浓度（500 mg L〜（-1））下重复使用生物催化剂，可以使我们获得9个循环。

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