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首页> 外文期刊>Journal of industrial and engineering chemistry >Investigation the activity and stability of lysozyme on presence of magnetic nanoparticles
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Investigation the activity and stability of lysozyme on presence of magnetic nanoparticles

机译：研究磁性纳米粒子存在下溶菌酶的活性和稳定性

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In this work the influence of Fe2O3 magnetic nanoparticle on lysozyme activity and thermal stability were investigated. The effect of nano-Fe2O3 on lysozyme activity was studied by UV-vis spectrophotometry at 35 degrees C in pH = 7.25 using sodium phosphate as a buffer. Measurements were carried out using 6 x 10(-8) M (0.1 mg/ml) of lysozyme and a range of nano-Fe2O3 concentrations between 0 and 0.045 mg/ml. It was found that by increasing the concentration of nano-Fe2O3, the rate of Micrococcus lysodeikticus lyses (lysozyme activity) will be increased. On the other hand, the thermal stability of lysozyme was investigated in the presence of nano-Fe2O3 over the temperature range (from 293 K to 363 K). Results indicate that by increasing of nano-Fe2O3, the thermal stability of lysozyme will be increased. (C) 2014 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
机译：在这项工作中，研究了Fe2O3磁性纳米颗粒对溶菌酶活性和热稳定性的影响。纳米Fe2O3对溶菌酶活性的影响是通过在35℃，pH = 7.25，使用磷酸钠作为缓冲液的紫外可见分光光度法研究的。使用6 x 10（-8）M（0.1 mg / ml）溶菌酶和0至0.045 mg / ml的纳米Fe2O3浓度进行测量。已经发现，通过增加纳米Fe 2 O 3的浓度，溶菌微球菌裂解率（溶菌酶活性）将增加。另一方面，研究了在温度范围（293 K至363 K）下存在纳米Fe2O3时溶菌酶的热稳定性。结果表明，通过增加纳米Fe2O3，溶菌酶的热稳定性将会提高。 （C）2014大韩工业化学工程学会。由Elsevier B.V.发布。保留所有权利。

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