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Chitin oligomers and monomers production by coupling gamma radiation and enzymatic hydrolysis

机译:偶联伽马射线和酶促水解生产几丁质低聚物和单体

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The main purpose of this study was the chitin enzymatic hydrolysis enhancement by a pretreatment, consisting of chitin exposure to gamma radiation at several doses, ranging from 15 kGy to 210 kGy. The solid chitin molecular weight reduction achieved by Co-60 gamma rays treatment reached a 90% rate at the t used, with a dose of 210 kGy. The partial depolymerization of chitin was realized by irradiation allowing an enzyme affinity increase toward this substrate. The monomers and oligomers obtained from their radiated chitin production rate were almost 100%. It was also demonstrated that the NAG production by enzymatic hydrolysis of irradiated chitin at 210 Gy follows Michaelis-Menton kinetics. (C) 2014 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
机译:这项研究的主要目的是通过预处理增强几丁质酶水解,包括几丁质在15 kGy至210 kGy的几剂量下暴露于伽马射线。通过Co-60伽玛射线处理实现的固体几丁质分子量降低在所使用的t处达到90%的速率,剂量为210 kGy。几丁质的部分解聚是通过照射实现的,从而允许对该底物的酶亲和力增加。由其辐射的几丁质产生率获得的单体和低聚物几乎为100%。还证明了通过210 Gy的辐照甲壳质的酶促水解产生的NAG遵循Michaelis-Menton动力学。 (C)2014大韩工业化学工程学会。由Elsevier B.V.发布。保留所有权利。

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