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首页> 外文期刊>Journal of Invertebrate Pathology >Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp
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Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp

机译：表现出白色粪便综合征的南美白对虾粪便中的小孢子虫肝细胞肝炎（EHP）致密种群及其向健康虾的传播途径

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摘要

White feces syndrome (WFS) is an emerging problem for penaeid shrimp farming industries in SE Asia countries, Thailand, Malaysia, Vietnam, Indonesia, China, and in India. This occurrence of this syndrome is usually first evidenced by the appearance of white fecal strings floating on surface of the shrimp ponds. The gross signs of affected shrimp include the appearance of a whitish hindgut and loose carapace, and it is associated with reduced feeding and growth retardation. To investigate the nature of the white feces syndrome, samples of white feces and shrimp hepatopancreas tissue were collected from Penaeus vannamei in affected farms in Indonesia, and these were examined histologically. Within the white feces, we found densely packed spores of the microsporidian Enterocytozoon hepatopenaei (abbreviated as EHP) and relatively fewer numbers of rod-shaped bacteria. From WFS ponds, hepatopancreas samples form 30 individual shrimp were analyzed by histology and in situ hybridization. The results showed that all of the shrimp examined were infected with EHP accompanied by septic hepatopancreatic necrosis (SHPN). Midgut epithelial cells were also infected and this increased the number of tissue types being affected by EHP. By PCR, EHP was detected in all the samples analyzed from WFS-affected ponds, but not in those sampled from healthy shrimp ponds. To determine the modes of transmission for this parasite, we performed feeding and cohabitation bioassays, the results showed that EHP can be transmitted through per os feeding of EHP-infected hepatopancreas tissue to healthy shrimp and through cohabitation of infected and healthy shrimp. In addition, we found the use of Fumagillin-B, an antimicrobial agent, was ineffective in either reducing or eliminating EHP in infected shrimp. (C) 2016 Elsevier Inc. All rights reserved.
机译：在东南亚国家，泰国，马来西亚，越南，印度尼西亚，中国和印度，对虾养殖业中存在白色屎综合征（WFS）。通常首先通过虾池表面漂浮的白色粪便串来证明这种综合征的发生。受影响虾的总体体征包括后肠发白和甲壳松弛，与减少的摄食和生长迟缓有关。为了调查白色粪便综合征的性质，从印度尼西亚受影响的南美白对虾中收集了白色粪便和虾肝胰腺组织的样本，并对其进行了组织学检查。在白色的粪便中，我们发现了小孢子虫肝细胞小孢子虫肝小孢子菌（简称EHP）的密集孢子和相对较少数量的杆状细菌。从WFS池塘中，通过组织学和原位杂交分析了30只虾的肝胰腺样本。结果表明，所有检查的虾均感染了EHP，并伴有败血性肝胰腺坏死（SHPN）。中肠上皮细胞也被感染，这增加了受EHP影响的组织类型的数量。通过PCR，在从世界粮食首脑会议（WFS）影响的池塘中分析的所有样品中均检测到了EHP，但在健康虾池中未检出。为了确定这种寄生虫的传播方式，我们进行了饲喂和同居生物测定，结果表明，EOS可以通过经口饲喂被EHP感染的肝胰腺组织传播给健康虾，以及通过被感染和健康虾的同居而传播。此外，我们发现使用抗细菌剂Fumagillin-B不能有效减少或消除感染虾中的EHP。 （C）2016 Elsevier Inc.保留所有权利。

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