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首页> 外文期刊>Journal of Lipid Research >Oxidized LDL induces the expression of ALBP/aP2 mRNA and protein in human THP-1 macrophages
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Oxidized LDL induces the expression of ALBP/aP2 mRNA and protein in human THP-1 macrophages

机译：氧化的低密度脂蛋白诱导人THP-1巨噬细胞中ALBP / aP2 mRNA和蛋白的表达

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The adipocyte lipid-binding protein (ALBP/aP2) belongs to a multigene family of fatty acid and retinoid transport proteins. This protein is abundantly expressed in the cytoplasm and nuclear region of adipocytes and is postulated to serve as a lipid shuttle, solubilizing hydrophobic fatty acids and delivering them to the appropriate metabolic system for utilization. This report demonstrates that human cholesterol-loaded THP-1 macrophages express ALBP/aP2 and that its expression can be stimulated by oxidized low density lipoprotein (oxLDL). The increase in mRNA expression was paralleled by a similar increase in ALBP/aP2 protein. The increase in ALBP/aP2 mRNA and protein in oxLDL-stimulated THP-1 macrophages is concentration and time dependent and is inhibited by treatment of the cells with an antioxidant inhibitor of nuclear factor-kappaB (NF-kappaB), pyrrolidine dithiocarbamate (PDTC), and with protein kinase C (PKC) inhibitors bisindolylmaleimide I and Ro-31-8220. These results suggest that activation of both NF-kappaB and PKC signaling pathways is necessary for oxLDL- induced ALBP/aP2 gene expression in THP-1 macrophages and that the upregulation of the fatty acid carrier may be a necessary event in foam cell formation. [References: 49]
机译：脂肪细胞脂质结合蛋白（ALBP / aP2）属于脂肪酸和类维生素A转运蛋白的多基因家族。该蛋白质在脂肪细胞的细胞质和核区域中大量表达，并被假定为可作为脂质穿梭物，溶解疏水性脂肪酸并将其输送至适当的代谢系统以供利用。该报告证明载人胆固醇的THP-1巨噬细胞表达ALBP / aP2，并且其表达可以被氧化的低密度脂蛋白（oxLDL）刺激。 mRNA表达的增加与ALBP / aP2蛋白的类似增加平行。 oxLDL刺激的THP-1巨噬细胞中ALBP / aP2 mRNA和蛋白的增加是浓度和时间依赖性的，并且可以通过用核因子-κB（NF-kappaB），吡咯烷二硫代氨基甲酸酯（PDTC）的抗氧化剂抑制细胞来抑制，以及蛋白激酶C（PKC）抑制剂bisindolylmaleimide I和Ro-31-8220。这些结果表明，NF-κB和PKC信号通路的激活对于oxLDL诱导的THP-1巨噬细胞中的ALBP / aP2基因表达是必需的，并且脂肪酸载体的上调可能是泡沫细胞形成中的必要事件。 [参考：49]

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