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首页> 外文期刊>Journal of Lipid Research >A sandwich enzyme-linked immunosorbent assay for human serum paraoxonase concentration.
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A sandwich enzyme-linked immunosorbent assay for human serum paraoxonase concentration.

机译：用夹心酶联免疫吸附法测定人血清对氧磷酶的浓度。

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Serum paraoxonase (PON) is associated with plasma high density lipoproteins, and prevents the oxidative modification of low density lipoproteins. We have developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA), using two monoclonal antibodies against PON, to measure serum PON concentration. The concentration of PON in healthy Japanese subjects was 59.3 +/- 1.3 microgram/mL (mean +/- SEM; n = 87). Serum PON concentrations in relation to the PON 192 genetic polymorphism were: 69.5 +/- 2.9 microgram/mL in the QQ genotype; 63.0 +/- 1.9 microgram/mL in the QR genotype; and 52.8 +/- 1.7 microgram/mL in the RR genotype. Concentrations were significantly lower in the RR than in the QQ genotype (P < 0.01). Serum paraoxonase specific activity was higher in RR than in QQ subjects (18.6 +/- 0.40 vs. 2. 56 +/- 0.05 nmol/min/microgram, P < 0.01), but arylesterase specific activity was unrelated to genotype. PON concentration was positively associated (P < 0.001) with both serum arylesterase activity and, after adjusting for the effect of the position 192 polymorphism, with serum paraoxonase activity. Subjects with angiographically verified coronary heart disease had significantly lower PON concentrations than the healthy controls (52.0 +/- 2.3 microgram/mL; n = 35, P < 0.01). This association was independent of the position 192 genotype. Our new ELISA should be of value for epidemiologic and clinical studies of serum PON concentration. immunosorbent assay for human serum paraoxonase concentration.
机译：血清对氧磷酶（PON）与血浆高密度脂蛋白相关，并防止低密度脂蛋白的氧化修饰。我们已经开发了一种灵敏的三明治酶联免疫吸附测定（ELISA），使用两种针对PON的单克隆抗体来测量血清PON浓度。健康日本受试者中PON的浓度为59.3 +/- 1.3微克/ mL（平均值+/- SEM; n = 87）。与PON 192基因多态性相关的血清PON浓度为：QQ基因型为69.5 +/- 2.9微克/毫升; QR基因型为63.0 +/- 1.9微克/ mL; RR基因型为52.8 +/- 1.7微克/ mL。 RR中的浓度显着低于QQ基因型（P <0.01）。 RR中的血清对氧磷酶比活性高于QQ受试者（18.6 +/- 0.40对2. 56 +/- 0.05 nmol / min / microgram，P <0.01），但芳基酯酶比活性与基因型无关。 PON浓度与血清芳基酯酶活性呈正相关（P <0.001），并且在调整192位多态性的影响后，与血清对氧磷酶活性呈正相关（P <0.001）。经血管造影证实为冠心病的受试者的PON浓度明显低于健康对照组（52.0 +/- 2.3微克/毫升; n = 35，P <0.01）。这种关联与位置192基因型无关。我们的新ELISA对血清PON浓度的流行病学和临床研究具有价值。人体血清对氧磷酶浓度的免疫吸附测定。

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