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首页> 外文期刊>Journal of Lipid Research >Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans.
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Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans.

机译：线粒体和过氧化物酶体靶向人类中的2-甲基酰基辅酶A消旋酶。

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摘要

2-Methylacyl-CoA racemase is an auxiliary enzyme required for the peroxisomal beta-oxidative breakdown of (2R)-pristanic acid and the (25R)-isomer of C(27) bile acid intermediates. The enzyme activity is found not only in peroxisomes but also is present in mitochondria of human liver and fibroblasts. The C terminus of the human racemase, a protein of 382 amino acids with a molecular mass of 43,304 daltons as deduced from its cloned cDNA, consists of KASL. Hitherto this sequence has not been recognized as a peroxisomal targeting signal (PTS1). From the in vitro interaction between recombinant racemase and recombinant human PTS1 receptor (Pex5p), and the peroxisomal localization of green fluorescent protein (GFP) fused to the N terminus of full-length racemase or its last six amino acids in tranfected Chinese hamster ovary (CHO) cells, we concluded that ASL is a new PTS1 variant. To be recognized by Pex5p, however, the preceding lysine residue is critical. As shown in another series of transfection experiments with GFP fused to the C terminus of the full-length racemase or racemase with deletions of the N terminus, mitochondrial targeting information is localized between amino acids 22 and 85.Hence, our data show that a single transcript gives rise to a racemase protein containing two topogenic signals, explaining the dual cellular localization of the activity.
机译：2-甲基酰基辅酶A消旋酶是过氧化物酶体β-氧化分解（2R）-链烷酸和C（27）胆汁酸中间体的（25R）-异构体所需的辅助酶。该酶活性不仅存在于过氧化物酶体中，而且存在于人肝和成纤维细胞的线粒体中。从其克隆的cDNA推导出，人消旋酶的C末端是382个氨基酸的蛋白质，分子量为43,304道尔顿。迄今为止，尚未将该序列识别为过氧化物酶体靶向信号（PTS1）。从重组消旋酶与重组人PTS1受体（Pex5p）的体外相互作用以及融合到全长消旋酶N末端或其最后六个氨基酸的绿色荧光蛋白（GFP）的过氧化物酶体定位（在转染的中国仓鼠卵巢中）（ CHO）细胞，我们得出结论，ASL是一个新的PTS1变体。但是，要被Pex5p识别，前面的赖氨酸残基至关重要。如另一系列转染实验所示，将GFP融合至全长消旋酶的C末端或缺失N末端的消旋酶，线粒体靶向信息位于22至85位氨基酸之间。转录物产生包含两个拓扑信号的消旋酶蛋白，解释了该活性的双重细胞定位。

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