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首页> 外文期刊>Journal of Lipid Research >Deletion of the propeptide of apolipoprotein A-I reduces protein expression but stimulates effective conversion of prebeta-high density lipoprotein to alpha-high density lipoprotein.
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Deletion of the propeptide of apolipoprotein A-I reduces protein expression but stimulates effective conversion of prebeta-high density lipoprotein to alpha-high density lipoprotein.

机译：载脂蛋白A-1的前肽的缺失降低了蛋白表达，但是刺激了前β-高密度脂蛋白向α-高密度脂蛋白的有效转化。

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摘要

The properties of the mature and pro-forms of recombinant apolipoprotein A-I (apoA-I) were compared with those of apoA-I isolated from human plasma. When the synthesis and secretion of pro- and mature forms of apoA-I from a baculovirus/insect cell expression system were compared in parallel experiments, the amount of the pro-form of apoA-I synthesized and secreted was severalfold higher than that of the mature form of apoA-I. A comparison of the properties of the pro- and mature forms of recombinant apoA-I and human plasma apoA-I showed no difference between all three in their secondary structure, their ability to self-associate, lipid-binding capacity, lecithin: cholesterol acyltransferase activation, and binding to the phospholipid transfer protein. The properties of reconstituted high density lipoprotein (HDL) particles formed from the proteins and their ability to promote cholesterol and phospholipid efflux from human skin fibroblasts were also similar. However, their ability to bind to plasma HDL subfractions differed, because twice as much proapoA-I associated with prebeta(1)-HDL and prebeta(2)-HDL subfractions compared with both mature recombinant and plasma apoA-I. Correspondingly, the amount of proapoA-I in alpha-HDL subfractions, especially in alpha(1)-HDL and alpha(2)-HDL, was decreased. We conclude that while the propeptide of apoA-I is required for the effective synthesis and secretion of apoA-I, cleavage of this peptide is a requisite for the effective interconversion of HDL subfractions.
机译：将重组载脂蛋白A-I（apoA-I）的成熟形式和原形式的特性与从人血浆中分离出的apoA-I的特性进行了比较。在平行实验中比较杆状病毒/昆虫细胞表达系统合成和分泌的apoA-I的前体和成熟形式时，合成和分泌的apoA-I的前体形式的量要比杆状病毒/昆虫细胞表达系统的高。 apoA-I的成熟形式。重组apoA-I和人血浆apoA-I的前，成熟形式的性质比较表明，这三个子实体的二级结构，自结合能力，脂质结合能力，卵磷脂：胆固醇酰基转移酶之间没有差异活化，并与磷脂转移蛋白结合。由蛋白质形成的重组高密度脂蛋白（HDL）颗粒的性质以及它们从人皮肤成纤维细胞中促进胆固醇和磷脂外排的能力也很相似。但是，它们结合血浆HDL亚组分的能力有所不同，因为与成熟重组和血浆apoA-I相比，与prebeta（1）-HDL和prebeta（2）-HDL亚组分相关的proapoA-I含量要高两倍。相应地，减少了α-HDL亚组分中proapoA-I的量，尤其是在α（1）-HDL和α（2）-HDL中。我们得出结论，尽管apoA-I的前肽是apoA-I的有效合成和分泌所必需的，但该肽的裂解是HDL亚组分有效相互转化的必要条件。

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