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首页> 外文期刊>Journal of Lipid Research >Regioisomeric distribution of cholesteryl linoleate hydroperoxides and hydroxides in plasma from healthy humans provides evidence for free radical-mediated lipid peroxidation in vivo.
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Regioisomeric distribution of cholesteryl linoleate hydroperoxides and hydroxides in plasma from healthy humans provides evidence for free radical-mediated lipid peroxidation in vivo.

机译：健康人血浆中胆固醇亚油酸氢过氧化物和氢氧化物的区域异构分布为体内自由基介导的脂质过氧化提供了证据。

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摘要

We have previously reported the detection of cholesteryl ester hydroperoxides, consisting mainly of cholesteryl linoleate hydroperoxides (Ch18:2-OOH), at nm levels in plasma from healthy humans (Y. Yamamoto and E. Niki, 1989. Biochem. Biophys. Res. Commun. 165: 988-993). To elucidate their production mechanism in vivo, we examined the distribution of Ch18:2-O(O)H regioisomers in blood plasma from nine healthy young subjects using a sequential method consisting of methanol/hexane extraction in the presence of antioxidant, reductant, and internal standard, solid phase extraction to remove unoxidized cholesteryl linoleate, purification by reversed-phase high-performance liquid chromatography (HPLC), and detection by normal phase HPLC. Furthermore, we confirm that little artifactual oxidation of cholesteryl linoleate occurred during analytical procedures indicated by the absence of oxidation products of cholesteryl 11Z,14Z-eicosadienoate (Ch20:2) when provided as an exogenous substrate to the experimental procedure. We detected nm levels of all free radical-mediated oxidation products, 13ZE-, 13EE-, 9-EZ-, and 9-EE-forms of Ch18:2-O(O)H, in blood plasma, whereas the 13ZE-isomer resulting from enzymatic 15-lipoxygenase oxidation was not evident as a major product. These results indicate that free radical chain oxidation of lipids occurs even in healthy young individuals.
机译：我们先前已经报道了在健康人血浆中以nm水平检测到胆固醇酯氢过氧化物，其主要由胆甾醇亚油酸酯氢过氧化物（Ch18：2-OOH）组成（Y. Yamamoto and E. Niki，1989. Biochem。Biophys。Res。 165：988-993）。为了阐明其体内产生机理，我们使用了由抗氧化剂，还原剂和抗氧化剂在甲醇/己烷中提取的顺序方法，对9名健康年轻受试者血浆中Ch18：2-O（O）H区域异构体的分布进行了研究。内标，固相萃取以去除未氧化的胆固醇亚油酸酯，通过反相高效液相色谱（HPLC）纯化，并通过正相HPLC检测。此外，我们确认当作为实验程序的外源底物提供时，由于没有胆固醇基11Z，14Z-二十碳二烯酸酯（Ch20：2）的氧化产物的存在，在分析程序中几乎没有人为地氧化胆固醇基亚油酸酯。我们检测到血浆中所有自由基介导的氧化产物，Ch18：2-O（O）H的13ZE-，13EE-，9-EZ-和9-EE-形式的nm水平，而13ZE-异构体酶促15-脂氧合酶氧化产生的产物并不明显。这些结果表明，即使在健康的年轻个体中，脂质的自由基链氧化也会发生。

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