...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Lipid Research >Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response.
【24h】

Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response.

机译：通过ESI-MS定量测定磷脂成分：酰基链长，不饱和度和脂质浓度对仪器响应的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Electrospray ionization-mass spectrometry (ESI-MS) is a very promising tool for the analysis of phospholipid compositions, but is hampered by the fact that not all molecular species are detected with equal efficiency. We studied this and other issues that need to be taken into account to obtain truly quantitative compositional data. The key findings were as follows: First, the instrument response for both saturated and unsaturated phospholipid species decreased with increasing acyl chain length. This effect became increasingly prominent with increasing overall lipid concentration. Second, the degree of acyl chain unsaturation also had a significant effect on instrument response. At the highest concentration studied (10 pmol/microl), polyunsaturated species gave 40% higher intensity than the fully saturated ones. The effect of unsaturation diminished and nearly disappeared with progressive dilution. Third, the instrument response for the different head group classes varied markedly depending on the infusion solvent used. Notably, inclusion of ammonia in the infusion solvent eliminated sodium adduct formation in the positive ion mode, thus greatly simplifying the interpretation of the spectra. The fact that instrument response is dependent on many structural features, overall lipid concentration, solvent composition, and instrument settings makes it necessary to include several internal standards for each phospholipid class to obtain accurate data. Preferably, both unsaturated and saturated standards should be used. Finally, we quantified the major phospholipid classes of BHK cells using ESI-MS. The data agreed closely with those obtained with thin-layer chromatography and phosphorus analysis. This study indicates that quantitative compositional data can be obtained with ESI-MS, provided that proper attention is paid to experimental details, particularly the choice of internal standards.
机译：电喷雾电离质谱（ESI-MS）是用于分析磷脂成分的非常有前途的工具，但是由于并非所有分子种类都以相同的效率被检测的事实而受到阻碍。我们研究了此问题和其他一些需要考虑的问题，以获得真正的定量组成数据。主要发现如下：首先，随着酰基链长度的增加，饱和和不饱和磷脂种类的仪器响应均降低。随着总脂质浓度的增加，这种作用变得越来越突出。其次，酰基链不饱和度对仪器响应也有重要影响。在研究的最高浓度（10 pmol /μl）下，多不饱和物质的强度比完全饱和物质高40％。随着进行性稀释，不饱和的作用减弱并且几乎消失。第三，对于不同的头组类别，仪器的响应随所使用的输注溶剂而显着不同。值得注意的是，在输注溶剂中包含氨消除了正离子模式下钠加合物的形成，因此大大简化了光谱的解释。仪器响应取决于许多结构特征，总体脂质浓度，溶剂组成和仪器设置的事实，因此有必要为每种磷脂类别包括多个内标以获得准确的数据。最好同时使用不饱和标准液和饱和标准液。最后，我们使用ESI-MS量化了BHK细胞的主要磷脂类别。数据与通过薄层色谱和磷分析获得的数据非常吻合。这项研究表明，只要适当注意实验细节，尤其是内标的选择，就可以使用ESI-MS获得定量的成分数据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号