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首页> 外文期刊>Journal of Lipid Research >Lipoprotein assembly capacity of the mammary tumor-derived cell line C127 is due to the expression of functional microsomal triglyceride transfer protein.
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Lipoprotein assembly capacity of the mammary tumor-derived cell line C127 is due to the expression of functional microsomal triglyceride transfer protein.

机译：乳腺肿瘤衍生细胞系C127的脂蛋白装配能力是由于功能性微粒体甘油三酸酯转移蛋白的表达所致。

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摘要

C127, a murine mammary tumor-derived cell line, is capable of lipidating and secreting apolipoprotein B-41 (apoB-41) in the apparent absence of microsomal triglyceride transfer protein (MTP). Using a semiquantitative reverse transcriptase-coupled polymerase chain reaction, mouse MTP mRNA was detected in C127 cells at approximately 10-20% of the relative abundance of human MTP in HepG2 cells. Radiolabeling of C127 cells with [35S]methionine and [35S]- cysteine followed by immunoprecipitation with anti-MTP antibodies identified a band with an electrophoretic mobility identical to that of authentic mouse MTP. Cotransfection of apoB-41 and the MTP 97-kDa subunit in C127 cells enhanced apoB secretion by approximately 5-fold relative to apoB-41 transfection alone, suggesting that MTP is limiting in these cells. To establish that MTP expression is responsible for apoB-containing lipoprotein assembly in C127 cells, the effects of the MTP inhibitor BMS-200150 were examined. Secretion of apoB-41 by C127 cells was inhibited to the same extent observed in COS-1 cells cotransfected with apoB-41 and MTP. These results suggest that low MTP expression, and not the expression or overexpression of another known or novel factor(s), is responsible for apoB assembly and secretion in C127 cells and further supports the essential nature of MTP in the biogenesis of apoB-containing lipoproteins. .
机译：C127是鼠类乳腺肿瘤衍生的细胞系，在明显不存在微粒体甘油三酸酯转移蛋白（MTP）的情况下，能够脂化并分泌载脂蛋白B-41（apoB-41）。使用半定量逆转录酶偶联的聚合酶链反应，在C127细胞中检测到的小鼠MTP mRNA约为HepG2细胞中人MTP相对丰度的10-20％。用[35S]蛋氨酸和[35S]-半胱氨酸对C127细胞进行放射性标记，然后用抗MTP抗体进行免疫沉淀，从而鉴定出一条电泳迁移率与真实小鼠MTP相同的谱带。与单独的apoB-41转染相比，C127细胞中apoB-41和MTP 97-kDa亚基的共转染使apoB分泌提高了约5倍，这表明MTP在这些细胞中是限制性的。为了确定MTP表达是导致C127细胞中含apoB的脂蛋白组装的原因，研究了MTP抑制剂BMS-200150的作用。在用apoB-41和MTP共转染的COS-1细胞中，C127细胞对apoB-41的分泌受到的抑制程度相同。这些结果表明，MTP的低表达而不是另一种已知或新因子的表达或过表达，是C127细胞中apoB组装和分泌的原因，并进一步支持MTP在含apoB的脂蛋白的生物发生中的本质性质。 。 。

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