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首页> 外文期刊>Journal of Lipid Research >A novel gene conserved from yeast to humans is involved in sterol biosynthesis.
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A novel gene conserved from yeast to humans is involved in sterol biosynthesis.

机译：从酵母到人类保守的新基因参与固醇的生物合成。

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摘要

The ERG28 gene was originally identified by microarray expression profiling as possibly involved in the Saccharomyces cerevisiae sterol pathway. Microarray analyses suggested that the transcription pattern of ERG28 closely followed that of genes involved in sterol synthesis. ERG28 was also found in Schizosaccharomyces pombe and Arabidopsis as well as humans, and in the latter was shown to be highly expressed in adult testis tissue. All four proteins contain potential transmembrane domain(s). Gas chromatography-mass spectrometry analysis of an ERG28-deleted S. cerevisiae strain (which is slow growing but not auxotrophic for ergosterol) indicates a lesion in sterol C-4 demethylation. Sterol profiles indicate accumulation of 3-keto and carboxylic acid sterol intermediates, which are involved in removing the two C-4 methyl groups from the sterol A ring. Similar intermediates have previously been demonstrated to accumulate in erg26 (sterol dehydrogenase/decarboxylase) and erg27 (3-ketoreductase) mutants in yeast.We speculate that the role of the Erg28 protein (Erg28p) may be either to tether Erg26p and Erg27p to the endoplasmic reticulum or to facilitate interaction between these proteins.-Gachotte, D., J. Eckstein, R. Barbuch, T. Hughes, C. Roberts, and M. Bard. A novel gene conserved from yeast to humans is involved in sterol biosynthesis. J. Lipid Res. 2001. 42: 150;-154.
机译：ERG28基因最初是通过微阵列表达谱鉴定的，可能与酿酒酵母固醇途径有关。芯片分析表明，ERG28的转录模式紧随固醇合成相关基因的转录模式。在裂殖酵母和拟南芥以及人类中也发现了ERG28，后者在成人睾丸组织中被高度表达。所有四种蛋白质均含有潜在的跨膜结构域。缺失ERG28的酿酒酵母菌株（其生长缓慢但麦角固醇不是营养缺陷型）的气相色谱-质谱分析表明，固醇C-4脱甲基有损伤。甾醇曲线表明3-酮和羧酸固醇中间体的积累，这涉及从固醇A环上除去两个C-4甲基。先前已证明类似的中间体会在酵母中的erg26（固醇脱氢酶/脱羧酶）和erg27（3-酮还原酶）突变体中积累。我们推测Erg28蛋白（Erg28p）的作用可能是将Erg26p和Erg27p系于内质网或促进这些蛋白质之间的相互作用。-Gachotte，D.，J.Eckstein，R.Barbuch，T.Hughes，C.Roberts和M.Bard。从酵母到人类保守的新基因参与固醇的生物合成。 J.脂质研究。 2001.42：150; -154。

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