...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Korean Forestry Society >Isolation and Identification of Fusicoccum Species from Quercus dentata
【24h】

Isolation and Identification of Fusicoccum Species from Quercus dentata

机译：齿状栎镰刀菌属物种的分离与鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An imperfect fungus Fusiococcum species was isolated from Quercus dentata. A naturally infected Daimyo oak tree was collected and showed elongate wounds on the stem. The fungal cultures were initially white and cottony, and later turned dark gray. Numerous solitary pycnidia were developed on the medium surface, and typically spherical. Yellowish conidial masses were exuded from pycnidia on the culture plates. Conidial masses were swollen and measured as approximately 100 to 300 um in length. It appeared that conidia were usually held together in globose to oval drops. Conidia were hyaline, single-celled (nonseptate), ellipsoid to fusoid, and measured as approximately 8.0x2.7 um. Based on these cultural and morphological characteristics, the fungal isolate was identified as a species of Fusicoccum Corda. To preserve and examine fungal spores exuded from pycnidia on the medium surface, a vapor fixation procedure for scanning electron microscopy was employed in this study. The specimens were exposed to the vapor of 2% (v/v) glutaraldehyde and 2% (w/v) osmium tetroxide each for 2 h. With the vapor fixation we obtained excellent retention of conidial masses in this study. The simple and versatile procedure for demonstrating fungal spores and their exudation from fruiting bodies would facilitate characterization of diverse pathological and environmental isolates as they are in native environments.
机译：从齿栎中分离出不完善的真菌镰刀菌。收集了自然感染的大名栎树，并在茎上显示出细长的伤口。真菌培养物最初是白色和棉质的，后来变成了深灰色。在中等表面上形成了许多孤立的pynicidia，通常为球形。培养皿上的吡虫啉渗出淡黄色的分生孢子团。分生孢子肿胀，长度约为100至300 um。似乎分生孢子通常以球状到椭圆形的液滴结合在一起。分生孢子是透明的，单细胞的（非分隔的），椭圆形到融合的，测量尺寸约为8.0x2.7 um。基于这些文化和形态特征，该真菌分离株被鉴定为镰刀菌的一种。为了保存和检查在培养基表面上从吡虫啉渗出的真菌孢子，在本研究中采用了用于扫描电子显微镜的蒸气固定程序。将样品分别暴露于2％（v / v）的戊二醛和2％（w / v）的四氧化蒸气中2小时。在本研究中，通过蒸汽固结，我们获得了极佳的分生孢子团保留率。证明真菌孢子及其从子实体中渗出的简单而通用的程序将有助于表征原生环境中各种病理和环境分离株。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号