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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Contribution of phopholipase D and a brefeldin A-sensitive ARF to chemoattractant-induced superoxide production and secretion of human neutrophils.
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Contribution of phopholipase D and a brefeldin A-sensitive ARF to chemoattractant-induced superoxide production and secretion of human neutrophils.

机译：磷脂酶D和布雷菲德菌素A敏感的ARF对趋化因子诱导的超氧化物生成和人类嗜中性白细胞分泌的贡献。

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摘要

We show that blockers of phospholipase D (PLD) reduce fMLP-triggered exocytosis of secretory vesicles effectively. In accordance with this, the PLD product phosphatidic acid (PA) was able to induce mobilization of secretory vesicles. Although PLD seems to play a role in the release of all neutrophil granule types, exogenous PA alone was not sufficient to activate the exocytosis of primary and secondary granules, suggesting that in the case of these granules, additional signaling factors are required to initiate the secretory responses. The ADP-ribosylation factor (ARF)-inhibitor brefeldin A (BFA) inhibited the fMLP-stimulated O2*- production strongly, whereas it did not influence any of the exocytic responses, and no significant effect of BFA was detected on the O2*- generation induced by other stimuli. On the basis of these results, we propose that upon chemoattractant stimulation, PLD activity is involved in induction of degranulation and O2*- production, but a BFA-sensitive ARF is only required to the activation of the NADPH oxidase. This ARF action seems to participate exclusively in the signaling pathway between the fMLP receptor and the oxidase.
机译：我们表明，磷脂酶D（PLD）的阻断剂有效地降低了fMLP触发的分泌性囊泡的胞吐作用。据此，PLD产物磷脂酸（PA）能够诱导分泌性囊泡的动员。尽管PLD似乎在所有中性粒细胞颗粒类型的释放中都起作用，但是仅外源PA不足以激活初级和次级颗粒的胞吐作用，这表明在这些颗粒的情况下，需要其他信号因子来启动分泌回应。 ADP核糖基化因子（ARF）抑制剂布雷菲德菌素A（BFA）强烈抑制fMLP刺激的O2 *-产生，但它不影响任何胞外反应，并且未检测到BFA对O2 *-有显着影响。其他刺激引起的产生。根据这些结果，我们建议在化学吸引剂刺激下，PLD活性与脱粒和O2 *-产生的诱导有关，但是BFA敏感的ARF仅对NADPH氧化酶的激活有效。这种ARF作用似乎仅参与fMLP受体和氧化酶之间的信号传导途径。

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