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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Rat monocyte-derived dendritic cells function and migrate in the same way as isolated tissue dendritic cells.
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Rat monocyte-derived dendritic cells function and migrate in the same way as isolated tissue dendritic cells.

机译：大鼠单核细胞衍生的树突细胞的功能和迁移与分离的组织树突细胞相同。

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摘要

Dendritic cells (DC) are the most potent antigen-presenting cells and are therefore useful to induce immune responses against tumor cells in patients. DC can be generated in vitro from monocytes using GM-CSF and IL-4, the so-called monocyte-derived DC (MoDC). To achieve antitumor responses, MoDC must be able to migrate to the draining lymph nodes after injection to induce cytotoxic T cells. Therefore, we studied migration of MoDC in a rat model. Functional rat MoDC were generated from PVG-RT7B rats and injected subcutaneously into PVG rats. These rat strains differ only at one epitope of the leukocyte-common antigen, which can be recognized by the antibody His 41. The advantage is that migrated cells can be detected in the draining lymph nodes by staining sections with His 41+; thus, migration is not influenced by labeling procedures. Rat MoDC migrated to the T-cell areas of the draining lymph nodes, just as isolated Langerhans cells or spleen DC do. In contrast, monocytes also migrated to the B-cell areas and the medulla.
机译：树突状细胞（DC）是最有效的抗原呈递细胞，因此可用于诱导患者体内针对肿瘤细胞的免疫反应。可以使用GM-CSF和IL-4从单核细胞体外产生DC，即所谓的单核细胞衍生DC（MoDC）。为了获得抗肿瘤反应，MoDC必须能够在注射后迁移至引流淋巴结以诱导细胞毒性T细胞。因此，我们在大鼠模型中研究了MoDC的迁移。从PVG-RT7B大鼠中产生功能性大鼠MoDC，并将其皮下注射到PVG大鼠中。这些大鼠品系仅在白细胞常见抗原的一个表位上有所不同，该表位可以被抗体His 41识别。优点是，通过用His 41+染色切片，可以在引流淋巴结中检测到迁移的细胞。因此，迁移不受标签程序的影响。大鼠MoDC迁移到引流淋巴结的T细胞区域，就像孤立的Langerhans细胞或脾DC一样。相反，单核细胞也迁移到B细胞区域和延髓。

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