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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Nitric oxide synthase and nitric oxide production in in vivo-derived mast cells.
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Nitric oxide synthase and nitric oxide production in in vivo-derived mast cells.

机译：体内肥大细胞中一氧化氮合酶和一氧化氮的产生。

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摘要

Nitric oxide (NO) is a potent mediator synthesized by a variety of cells involved in inflammatory reactions. We investigated the expression of NO synthase (NOS) in rat peritoneal mast cells (PMC). Small amounts of eNOS mRNA were detected basally, whereas neither mRNA for iNOS nor nNOS was detected in unstimulated PMC. Following stimulation by antigen, interferon-gamma (IFN-gamma), or anti-CD8 antibody, PMC up-regulated iNOS mRNA expression. In situ RT-PCR confirmed that iNOS mRNA originated from PMC. Production of iNOS protein was confirmed in stimulated PMC by immunohistochemistry. Upon stimulation with antigen, IFN-gamma, or anti-CD8, nitrite production was increased significantly (8.4+/-0.6, 7.6+/-0.9, and 6.6+/-0.9 microM/2x10(5) cells/48 h NO2-, respectively; P<0.01), whereas unstimulated PMC released 2.1 +/- 0.3 microM/2 x 10(5) cells/48 h NO2-. These findings demonstrate that in vivo-derived PMC transcribe and translate mRNA for NOS and produce NO.
机译：一氧化氮（NO）是由多种参与炎症反应的细胞合成的有效介体。我们调查了大鼠腹膜肥大细胞（PMC）中NO合酶（NOS）的表达。基本检测到少量的eNOS mRNA，而在未刺激的PMC中未检测到iNOS和nNOS的mRNA。在受到抗原，干扰素-γ（IFN-γ）或抗CD8抗体刺激后，PMC上调了iNOS mRNA表达。原位RT-PCR证实iNOS mRNA来源于PMC。通过免疫组织化学在刺激的PMC中证实了iNOS蛋白的产生。抗原，IFN-γ或抗CD8刺激后，亚硝酸盐的产生显着增加（8.4 +/- 0.6、7.6 +/- 0.9和6.6 +/- 0.9 microM / 2x10（5）细胞/ 48 h NO2-分别; P <0.01），而不受刺激的PMC释放2.1 +/- 0.3 microM / 2 x 10（5）细胞/ 48 h NO2-。这些发现表明，体内来源的PMC转录并翻译mRNA的NOS并产生NO。

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