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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Constitutive and regulated expression of platelet basic protein in human monocytes.
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Constitutive and regulated expression of platelet basic protein in human monocytes.

机译：人单核细胞中血小板碱性蛋白的组成型和调节型表达。

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摘要

Platelet basic protein (PBP) and several of its derivatives are known for their broad range of functions as signaling molecules and cationic antimicrobial peptides and were considered hitherto megakaryocyte- and platelet-specific. In search of glucocorticoid-regulated antimicrobial systems of monocytes, we found a 15-fold down-regulation of PBP mRNA by differential display. Regulation was confirmed in vivo even at low prednisone doses. Quantitative mRNA analyses confirmed down-regulation also for platelets. Western blotting and immunostains showed down-regulation at the protein level. Pro-PBP derivatives were in the size range of 7.5-14 kD and in immunostains, gave granular cytoplasmatic patterns. Interleukin (IL)-4 and IL-10 induced a similar down-regulation. Phagocytosis resulted in an increase of smaller derivatives in the range of 7.5 kD. Stimulation with interferon-gamma and lipopolysaccharide did decrease expression of PBP and affected derivatization. Expression of PBP and its derivatives is not restricted to the megakaryocytic cell lineage. PBP and some of its derivatives might contribute to the antimicrobial armamentarium of mononuclear phagocytes or have monokine functions. Our studies define PBPs as one among the many immunosuppressive targets of glucocorticoids.
机译：血小板碱性蛋白（PBP）及其几种衍生物以其广泛的信号传导分子和阳离子抗菌肽功能而闻名，迄今为止被认为是巨核细胞和血小板特异性的。在糖皮质激素调节的单核细胞抗菌系统中，我们通过差异显示发现了PBP mRNA的15倍下调。即使在强的松剂量低的情况下，体内调节仍得到确认。定量mRNA分析证实血小板也下调。 Western印迹和免疫染色显示蛋白质水平下调。 Pro-PBP衍生物的大小范围为7.5-14 kD，并在免疫染色中呈颗粒状细胞质模式。白介素（IL）-4和IL-10诱导类似的下调。吞噬作用导致较小的衍生物增加，范围为7.5 kD。干扰素-γ和脂多糖刺激确实降低了PBP的表达并影响了衍生作用。 PBP及其衍生物的表达不限于巨核细胞谱系。 PBP及其某些衍生物可能有助于单核吞噬细胞的抗菌武器或具有单因子功能。我们的研究将PBPs定义为糖皮质激素的许多免疫抑制靶标之一。

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