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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >High-level expression of I kappa B-beta in the surface epithelium of the colon: in vitro evidence for an immunomodulatory role.
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High-level expression of I kappa B-beta in the surface epithelium of the colon: in vitro evidence for an immunomodulatory role.

机译：IκB-β在结肠表面上皮中的高水平表达：免疫调节作用的体外证据。

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摘要

The intestinal epithelium is spatially segregated into two compartments, one containing undifferentiated cells in a proliferative state and one with non-proliferative differentiated cells. Although this epithelium can produce many immunemodulating substances, emerging evidence suggests that the differentiated cell compartment is less immune responsive. Indeed, it is the differentiated cellular compartment that represents the interface between the highly antigenic luminal environment and the mucosal immune system. The NF-kappaB/rel family of transcriptional activators play a critical role in regulating the inflammatory response by activating a wide variety of immune-modulating genes. These transcription factors are maintained in an inactive state in the cytoplasmic compartment by interaction with inhibitory proteins of the IkappaB family. In this study we show by immunohistochemistry that IkappaB-beta is expressed at high levels specifically in the differentiated surface epithelium of the colonic mucosa. Using a naturally occurring compound found in the colon of vertebrates, butyrate, we provide evidence in an intestinal cell line that alteration of IkappaB-beta expression can modulate the transcriptional activation of the interleukin-8 (IL-8) gene by preventing the nuclear translocation of NF-kappaB proteins. Therefore, the expression of IkappaB-beta in the differentiated surface epithelium of the colon may help these cells act as an immunological barrier to prevent activation of the mucosal immune system.
机译：肠上皮在空间上分隔成两个部分，一个部分包含处于增殖状态的未分化细胞，另一个部分包含非增殖分化细胞。尽管该上皮可以产生许多免疫调节物质，但新兴证据表明分化的细胞区室对免疫的反应较弱。实际上，正是分化的细胞区室代表了高抗原性腔环境与粘膜免疫系统之间的界面。 NF-kappaB / rel家族的转录激活因子通过激活多种免疫调节基因在调节炎症反应中发挥关键作用。这些转录因子通过与IkappaB家族的抑制蛋白相互作用而保持在细胞质区室的非活性状态。在这项研究中，我们通过免疫组织化学表明，IkappaB-beta在结肠黏膜的分化表面上皮中高水平表达。我们使用在脊椎动物结肠中发现的天然存在的化合物丁酸盐，在肠道细胞系中提供证据，证明IkappaB-β表达的改变可通过阻止核易位来调节白介素8（IL-8）基因的转录激活。 NF-κB蛋白的表达。因此，IkappaB-beta在结肠分化的表面上皮细胞中的表达可能有助于这些细胞充当免疫屏障，从而阻止粘膜免疫系统的激活。

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