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首页> 外文期刊>Journal of mass spectrometry: JMS >Comparison of CID, ETD and metastable atom-activated dissociation (MAD) of doubly and triply charged phosphorylated tau peptides
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Comparison of CID, ETD and metastable atom-activated dissociation (MAD) of doubly and triply charged phosphorylated tau peptides

机译：双电荷和三电荷磷酸化tau肽的CID，ETD和亚稳态原子活化解离（MAD）的比较

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摘要

The fragmentation behavior of the 2+ and 3+ charge states of eleven different phosphorylated tau peptides was studied using collision-induced dissociation (CID), electron transfer dissociation (ETD) and metastable atom-activated dissociation (MAD). The synthetic peptides studied contain up to two known phosphorylation sites on serine or threonine residues, at least two basic residues, and between four and eight potential sites of phosphorylation. CID produced mainly b-/y-type ions with abundant neutral losses of the phosphorylation modification. ETD produced c-/z-type ions in highest abundance but also showed numerous y-type ions at a frequency about 50% that of the z-type ions. The major peaks observed in the ETD spectra correspond to the charge-reduced product ions and small neutral losses from the charge-reduced peaks. ETD of the 2+ charge state of each peptide generally produced fewer backbone cleavages than the 3+ charge state, consistent with previous reports. Regardless of charge state, MAD achieved more extensive backbone cleavage than CID or ETD, while retaining the modification(s) in most cases. In all but one case, unambiguous modification site determination was achieved with MAD. MAD produced 15-20% better sequence coverage than CID and ETD for both the 2+ and 3+ charge states and very different fragmentation products indicating that the mechanism of fragmentation in MAD is unique and complementary to CID and ETD.
机译：使用碰撞诱导离解（CID），电子转移离解（ETD）和亚稳原子活化离解（MAD）研究了11种不同的磷酸化tau肽的2+和3+电荷状态的断裂行为。研究的合成肽在丝氨酸或苏氨酸残基上最多包含两个已知的磷酸化位点，至少两个碱性残基，并且在四个至八个潜在的磷酸化位点之间。 CID主要产生b- / y型离子，具有磷酸化修饰的大量中性损失。 ETD产生的c // z型离子丰度最高，但也显示出许多y型离子，其频率约为z型离子的50％。在ETD光谱中观察到的主要峰对应于减少电荷的产物离子和减少电荷的峰的中性损失。与以前的报道一致，每种肽的2+电荷状态的ETD通常比3+电荷状态产生更少的骨架裂解。不管电荷状态如何，MAD都比CID或ETD实现了更广泛的主链裂解，同时在大多数情况下保留了修饰。除了一种情况外，使用MAD可以实现明确的修饰位点确定。对于2+和3+电荷状态以及非常不同的片段化产物，MAD产生的序列覆盖率比CID和ETD好15-20％，这表明MAD中的片段化机制是独特的，并且与CID和ETD互补。

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