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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Co-expression of several human syntaxin genes in neutrophils and differentiating HL-60 cells: variant isoforms and detection of syntaxin 1.
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Co-expression of several human syntaxin genes in neutrophils and differentiating HL-60 cells: variant isoforms and detection of syntaxin 1.

机译：在嗜中性粒细胞和分化的HL-60细胞中共表达几种人类语法语法基因：变体亚型和语法语法1。

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摘要

Syntaxins are major components of vesicle trafficking and their pattern of expression depends on the cell type. Using reverse transcriptase-polymerase chain reaction (RT-PCR), cloning, and sequencing techniques, we have found that human neutrophils and neutrophil-differentiated HL-60 cells co-express syntaxins 1A, 3, 4, 5, 6, 7, 9, 11, and 16. These genes are also expressed in human peripheral blood lymphocytes and SH-SY5Y neuroblastoma cells, which, unlike neutrophils, also expressed syntaxin 10. We have identified two isoforms of syntaxin 3. Syntaxin 3A, similar to the previously reported syntaxin 3, and the novel isoform syntaxin 3B, which is identical to syntaxin 3A but lacks 37 amino acid residues at the carboxy-terminal region. Syntaxin 1 was mainly located to neutrophil granule membranes by confocal microscopy and by immunoblotting of subcellular fractions. These data indicate that syntaxin 1 cannot be considered specific to neural tissues. The level of expression of syntaxins 3, 4, 6, and 11 was increased during neutrophil differentiation of HL-60 cells, whereas that of syntaxins 1A, 5, 9, and 16 was unchanged. Syntaxin 7 was not expressed in undifferentiated HL-60 cells, but its expression was induced on neutrophil differentiation. The expression of several syntaxin genes in human neutrophils could be related to the high secretory capacity of these cells as well as to the presence of different cytoplasmic granules with distinct exocytic capabilities.
机译：语法素是囊泡运输的主要成分，其表达方式取决于细胞类型。使用逆转录酶-聚合酶链反应（RT-PCR），克隆和测序技术，我们发现人类嗜中性粒细胞和分化为中性粒细胞的HL-60细胞共表达语法素1A，3、4、5、6、7、9 ，11和16。这些基因还在人外周血淋巴细胞和SH-SY5Y神经母细胞瘤细胞中表达，与嗜中性粒细胞不同，SH-SY5Y神经母细胞瘤细胞也表达了syntaxin10。我们已经鉴定到了syntaxin 3的两种同工型。Syntaxin 3A，与先前报道的相似语法3和新的异构体语法3B，其与语法3A相同，但在羧基末端区域缺少37个氨基酸残基。通过共聚焦显微镜和通过亚细胞级分的免疫印迹，Syntaxin 1主要位于嗜中性粒细胞膜上。这些数据表明语法1不能被认为是特定于神经组织的。在HL-60细胞嗜中性粒细胞分化过程中，语法素3、4、6和11的表达水平增加，而语法素1A，5、9和16的表达水平不变。 Syntaxin 7在未分化的HL-60细胞中不表达，但其表达是在嗜中性粒细胞分化时诱导的。人类嗜中性粒细胞中几种syntaxin基因的表达可能与这些细胞的高分泌能力以及与具有不同胞外能力的不同细胞质颗粒的存在有关。

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