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首页> 外文期刊>Journal of mass spectrometry: JMS >Seed oil triglyceride profiling of thirty-two hybrid grape varieties
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Seed oil triglyceride profiling of thirty-two hybrid grape varieties

机译：32个杂交葡萄品种的种子油甘油三酸酯分析

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摘要

Triglyceride profile of seed oil samples from 32 hybrid grape varieties not studied before was investigated. A new method for the analysis of triacylglycerols (TAGs) has been developed based on the direct infusion in the electrospray ionization (ESI) source and employing tetrahydrofuran/methanol/ water (85:10:5 v|v|v) as solvent; the formation of [M + Na] ~+ ions in high yield has been observed. TAGs were identified by ESI-tandem mass spectrometry analysis, and the matrix-assisted-laser-desorption-ionization and time-of-flight profile of samples was determined. Six were the principal TAGs identified in seed oil: trilinolein (LLL) was the most abundant (43%), followed by dilinoleoyl-oleoylglycerol (LOL, 23%), and dilinoleoyl-palmitoylglycerol (LPL, 15%). Compounds present in lower concentration were LSL and LOO (11%), LOP (6%), and LSP (2%). Compared with seed oils produced from V. Vinifera grapes, some significant differences in the relative abundances of TAGs were found, in particular hybrid grape seed oils showed higher LOL and lower LPL content, respectively. Among the samples studied, a particularly high content of LLL (rich in unsaturated fatty acids) was found in seed oils from two red varieties.
机译：研究了从未研究过的32个杂交葡萄品种的种子油样品的甘油三酸酯谱。基于直接注入电喷雾电离（ESI）源并使用四氢呋喃/甲醇/水（85：10：5 v | v | v）作为溶剂，已经开发了一种分析三酰基甘油（TAGs）的新方法。已经观察到以高收率形成[M + Na]〜+离子。通过ESI串联质谱分析识别TAG，并确定样品的基质辅助激光解吸电离和飞行时间。六个是种子油中鉴定到的主要TAG：三亚麻油醇（LLL）含量最高（43％），其次是二亚麻油酰-油酰甘油（LOL，23％）和二亚麻油酰-棕榈酰甘油（LPL，15％）。浓度较低的化合物为LSL和LOO（11％），LOP（6％）和LSP（2％）。与V. Vinifera葡萄产生的种子油相比，TAGs的相对丰度有一些显着差异，特别是杂种葡萄籽油分别显示出较高的LOL和较低的LPL含量。在所研究的样品中，在两个红色品种的种子油中发现了特别高含量的LLL（富含不饱和脂肪酸）。

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