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首页> 外文期刊>Journal of mass spectrometry: JMS >Structural motifs in primary oxidation products of palmitoyl-arachidonoyl-phosphatidylcholines by LC-MS/MS
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Structural motifs in primary oxidation products of palmitoyl-arachidonoyl-phosphatidylcholines by LC-MS/MS

机译：LC-MS / MS分析棕榈酰-花生四烯酸-磷脂酰胆碱的初级氧化产物中的结构基序

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摘要

Oxidative modifications to phospholipids (OxPL) play a major role in modulating signaling events in inflammation and infection, and complete understanding on the induced biological effects can only be understood based on knowledge of the oxidative motifs present. Specific neutral losses observed in tandem mass spectrometry data (LC-MS/MS) of primary peroxidation products in oxidized palmitoyl-arachidonoyl-phosphatidylcholines (OxPAPC) provide information on the prevailing structural motifs regarding the oxidized acyl carbon chain, the nature of oxidized group and the site of carbon oxidation. The higher hydrophobicity of hydroperoxides compared to di-hydroxy derivatives under reverse-phase conditions together with specific fragmentation patterns enabled the identification of 12 structurally different OxPAPC structural (di-hydroxy and hydroperoxide derivatives) and positional isomers as well as the presence of poly-hydroxy together with isoprostanes derivatives. The fragmentation patterns described in quadrupole time-of-flight and linear ion trap instruments complement the m/z value and retention time parameters in the identification of oxidative composition in OxPAPC products becoming a valuable tool for the exploratory screening of oxidized phosphatidylcholines in OxPAPC extracts, distinction of native and modified PC isobaric structures in complex samples contributing to the increased understanding of redox lipidomics in inflammation and infection.
机译：磷脂的氧化修饰（OxPL）在调节炎症和感染中的信号事件中起主要作用，并且仅基于对存在的氧化基序的了解才能完全了解诱导的生物学效应。在串联的质谱数据（LC-MS / MS）中观察到的氧化过的棕榈酰-花生四烯酰基-磷脂酰胆碱（OxPAPC）中的主要过氧化产物的特定中性损失提供了有关氧化的酰基碳链，氧化的基团和碳氧化的位置。与反相条件下的二羟基衍生物相比，氢过氧化物的疏水性更高，并且具有特定的断裂模式，因此可以鉴定出12种结构上不同的OxPAPC结构（二羟基和氢过氧化物衍生物）和位置异构体，以及多羟基化合物的存在与异前列腺素衍生物一起。四极杆飞行时间和线性离子阱仪器中描述的碎片图谱与m / z值和保留时间参数互补，可鉴定OxPAPC产品中的氧化成分，成为探索性筛选OxPAPC提取物中的氧化磷脂酰胆碱的宝贵工具，天然和修饰的PC等压结构在复杂样品中的区别有助于增加对炎症和感染中氧化还原脂质组学的了解。

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