• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of mass spectrometry: JMS >Resolving double disulfide bond patterns in SNAP25B using liquid chromatography-ion trap mass spectrometry
【24h】

Resolving double disulfide bond patterns in SNAP25B using liquid chromatography-ion trap mass spectrometry

机译：液相色谱-离子阱质谱法分析SNAP25B中的双二硫键模式

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Complex disulfide bond patterns in synaptosomal-associated protein of 25 kD B (SNAP25B) are thought to regulate neurotransmitter release in response to oxidative stress. However, the steric feasibility of each possible disulfide pattern in SNAP25B has not been assessed. To assess the steric feasibility of hypothesized closely spaced complex disulfide patterning in SNAP25B and also the feasibility of identifying complex disulfide bond patterns with MS, we have developed a novel probabilistic analysis to unambiguously resolve complex double disulfide bond patterns by using an ion trap mass spectrometer. We analyzed fragmentation patterns of singly linked peptides to determine likely fragmentation events in an ion trap mass spectrometer and observed double and single backbone cleavage along with heterolytic cleavage of the disulfide bond. We modeled these same events in the doubly disulfide linked SNAP25B peptide and used a cumulative hypergeometric distribution with top-down scoring to both identify and differentiate these bonding patterns. Because of the presence of unique MS/MS peaks, two of the bonding patterns were directly identified. The third was assigned on the basis of full chromatographic separation and confirmed by modeling triple breakage fragments. In total, this work demonstrates the feasibility - and also limitations - of identification of complex intradisulfide patterns by using ion trap-based collision-induced dissociation-based fragmentation methods.
机译：突触体相关蛋白25 kD B（SNAP25B）中的复杂二硫键模式被认为可以调节神经递质的释放，以响应氧化应激。但是，尚未评估SNAP25B中每个可能的二硫键模式的空间可行性。为了评估SNAP25B中假设的紧密间隔的复杂二硫键图案的空间可行性以及使用MS识别复杂二硫键图案的可行性，我们开发了一种新型的概率分析，可以通过使用离子阱质谱仪明确地解决复杂的双二硫键图案。我们分析了单连接肽的片段化模式，以确定在离子阱质谱仪中可能发生的片段化事件，并观察到双，单主链裂解以及二硫键的杂合裂解。我们在双二硫键连接的SNAP25B肽中对这些相同事件进行了建模，并使用具有自上而下评分的累积超几何分布来识别和区分这些键合模式。由于存在独特的MS / MS峰，因此直接确定了两种键合模式。第三次基于完全色谱分离进行分配，并通过对三重断裂片段进行建模来确认。总的来说，这项工作证明了通过使用基于离子阱的碰撞诱导的基于离解的裂解方法鉴定复杂的二硫化物内谱图的可行性，以及局限性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号