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首页> 外文期刊>Journal of mass spectrometry: JMS >Simultaneous determination of three alkaloids, four ginsenosides and limonin in the plasma of normal and headache rats after oral administration of Wu-Zhu-Yu decoction by a novel ultra fast liquid chromatography-tandem mass spectrometry method: Application to a comparative pharmacokinetics and ethological study
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Simultaneous determination of three alkaloids, four ginsenosides and limonin in the plasma of normal and headache rats after oral administration of Wu-Zhu-Yu decoction by a novel ultra fast liquid chromatography-tandem mass spectrometry method: Application to a comparative pharmacokinetics and ethological study

机译：新型超快速液相色谱-串联质谱法同时测定吴茱-汤口服液正常和头痛大鼠血浆中三种生物碱，四种人参皂甙和柠檬苦素：在比较药代动力学和行为学研究中的应用

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摘要

A novel, sensitive and reliable ultra fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method has been developed and validated for simultaneous quantitation of eight main active ingredients (evodiamine, rutaecarpine, dehydroevodiamine, limonin, ginsenoside Rb1, Rd, Re and Rg1) in rat plasma after oral administration of Wu-Zhu-Yu (WZY) decoction, which is a celebrated and widely used Traditional Chinese Medicine formula for the treatment of headache. The analytes and internal standard (IS) were separated on a SHIM-PACK XR-ODS II column, and the detection was performed on a UFLC-MS/MS system with turbo ion spray source. The lower limits of quantification were 1.5, 0.5, 1.0, 2.0, 2.0, 1.0, 0.5 and 0.2 ng ml~(-1) for evodiamine, rutaecarpine, dehydroevodiamine, limonin, gensenoside Rb _1, Rd, Re and Rg_1, respectively. Linearity, accuracy, precision and absolute recoveries of the eight analytes were all within satisfaction. The IS-normalized matrix factor was adopted for assessing the matrix effect and accompanied with a satisfactory result. The validated method has been successfully applied to compare pharmacokinetic profiles of the eight active ingredients in rat plasma between normal and headache rats after administration. Exact pharmaceutical effect of WZY decoction on headache was demonstrated by the ethological response of headache rats induced by nitric oxide donor after administration. The results indicated that the absorption of evodiamine, rutaecarpine, gensenoside Rb_1, Re and Rg_1 in headache group were significantly higher than those in normal group with similar concentration-time curves while no significant differences existed in limonin and ginsenoside Rd between the two groups.
机译：开发了一种新颖，灵敏且可靠的超快速液相色谱-串联质谱（UFLC-MS / MS）方法，并验证了该方法可同时定量测定八种主要活性成分（依维他命，芸香果碱，脱氢戊二胺，柠檬苦素，人参皂苷Rb1，Rd，Re口服五指鱼汤后，大鼠血浆中的Rg1和Rg1）是一种著名的且广泛使用的中药配方，可用于治疗头痛。在SHIM-PACK XR-ODS II色谱柱上分离分析物和内标（IS），并在带有涡轮离子喷雾源的UFLC-MS / MS系统上进行检测。乙二胺，芸苔芸香碱，脱氢乙二胺，柠檬苦素，人参皂苷Rb_1，Rd，Re和Rg_1的定量下限分别为1.5、0.5、1.0、2.0、2.0、1.0、0.5和0.2 ng ml〜（-1）。八种分析物的线性，准确性，精密度和绝对回收率均令人满意。采用IS归一化的矩阵因子评估矩阵效果，并获得满意的结果。经验证的方法已成功应用于比较正常和头痛大鼠给药后大鼠血浆中八种活性成分的药代动力学特征。 WZY汤对头痛的确切药物作用由一氧化氮供体诱导的头痛大鼠在给药后的行为学反应证明。结果表明，头痛组中的evodiamine，rutaecarpine，人参皂甙Rb_1，Re和Rg_1的吸收均显着高于正常组，且浓度-时间曲线相似，而柠檬苦素和人参皂苷Rd的吸收之间无显着差异。

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