Application of ~(13)C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation

Salil Kumar Bhowmik; Vasanta Putluri; Ramakrishna Kommagani; Sai Aparna Konde; John P. Lydon; Arun Sreekumara; Nagireddy Putluri

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Application of ~(13)C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation

机译：液相色谱质谱法（LC-MS）〜（13）C同位素标记在确定含磷酸盐代谢结合中的应用

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Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with ~(13)C-labeled substrates. The method was applied to examine the ~(13)C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2-Deoxyglucose.

机译：在这里，我们描述了一种方法，其中负电喷雾电离质谱已用于了解在向细胞施用〜（13）C标记的底物后，通过含磷酸盐的代谢中间体的相对通量，该代谢中间体与中心碳代谢有关。该方法用于检查通过糖酵解在T47D乳腺癌细胞中〜（13）C的掺入，并显示在用2-脱氧葡萄糖处理后糖酵解相对通量降低。

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《Journal of mass spectrometry: JMS》 |2013年第12期|共6页
作者
Salil Kumar Bhowmik; Vasanta Putluri; Ramakrishna Kommagani; Sai Aparna Konde; John P. Lydon; Arun Sreekumara; Nagireddy Putluri;
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正文语种 eng
中图分类分析化学;
关键词
metabolic relative flux; single reaction monitoring; tandem mass spectrometry;

机译：代谢相对通量;单反应监测;串联质谱;

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1. Application of C-13 isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation (vol 48, pg 1270, 2013) [J] . Bhowmik Salil Kumar, Putluri Vasanta, Kommagani Ramakrishna, Journal of mass spectrometry: JMS . 2015,第10期

机译：使用液相色谱质谱法（LC-MS）进行C-13同位素标记在确定含磷酸盐的代谢结合中的应用（第48卷，第1270页，2013）
2. Application of ~(13)C stable isotope labeling liquid chromatography-multiple reaction monitoring-tandem mass spectrometry method for determining intact absorption of bioactive dipeptides in rats [J] . Nakashima E.M.N., Kudo A., Iwaihara Y., Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2011,第1期

机译：〜（13）C稳定同位素标记液相色谱-多反应监测-串联质谱法在大鼠生物活性二肽完整吸收测定中的应用
3. Characterization of metabolic profile of honokiol in rat feces using liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry and13C stable isotope labeling [J] . Yinfeng Dong, Minghai Tang, Hang Song, Journal of chromatography, B. Analytical technologies in the biomedical and life sciences . 2014,第Null期

机译：液相色谱结合四极杆飞行时间串联质谱和13 C稳定同位素标记表征大鼠粪便中厚朴酚的代谢谱
4. Low Labeling ~(13)C Metabolic Flux Analysis of Saccharomyces cerevisiae Using Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry [C] . Qi-ding Zhong, Guo-hui Li, Dong-dong Zhao, International conference on applied biotechnology . 2015

机译：啤酒酵母的低标记〜（13）C代谢通量的气相色谱-燃烧-同位素比质谱分析
5. Development of an assay for fatty acyl-CoAs using liquid chromatography-electrospray ionization-tandem mass spectrometry and its application to stable isotope-labeling and quantitation of sphingolipid metabolism [D] . Haynes, Christopher A. 2009

机译：液相色谱-电喷雾电离串联质谱法测定脂肪酰基辅酶A的研究及其在稳定同位素标记和鞘脂代谢定量中的应用
6. Determining bacteriophage endopeptidase activity using either fluorophore-quencher labeled peptides combined with liquid chromatography-mass spectrometry (LC-MS) or Förster resonance energy transfer (FRET) assays [O] . Rolf Lood, Henrik Molina, Vincent A. Fischetti 2012

机译：使用荧光猝灭剂标记的肽结合液相色谱-质谱（LC-MS）或Förster共振能量转移（FRET）测定法确定噬菌体内肽酶活性
7. Improved detection of honey adulteration by measuring differences between13C/12C stable carbon isotope ratios of protein and sugar compounds with a combination of elemental analyzer - isotope ratio mass spectrometry and liquid chromatography - isotope ratio mass spectrometry (δ13C-EA/LC-IRMS) [O] . Lutz Elflein, Kurt-Peter Raezke 2008

机译：通过元素分析仪-同位素比质谱和液相色谱-同位素比质谱（δ13C-EA/ LC-IRMS）的组合测量蛋白质和糖化合物的13C / 12C稳定碳同位素比率之间的差异，改进了对蜂蜜掺假的检测

Application of ~(13)C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation

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