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首页> 外文期刊>Journal of mass spectrometry: JMS >Optimal single-embryo mass spectrometry fingerprinting
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Optimal single-embryo mass spectrometry fingerprinting

机译：最佳单胚质谱指纹图谱

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摘要

In pre-implantation embryos, lipids play key roles in determining viability, cryopreservation and implantation properties, but often their analysis is analytically challenging because of the few picograms of analytes present in each of them. Matrix-assisted laser desorption/ionizationmass spectrometry (MALDI-MS) allows obtaining individual phospholipid profiles of thesemicroscopic organisms. This technique is sensitive enough to enable analysis of individual intact embryos and monitoring the changes in membrane lipid composition in the early stages of development serving as screening method for studies of biology and biotechnologies of reproduction. This article introduces an improved, more comprehensive MALDI-MS lipid fingerprinting approach that considerably increases the lipid information obtained from a single embryo. Using bovine embryos as a biologicalmodel, we have also tested optimal sample storage and handling conditions before the MALDI-MS analysis. Improved information at the molecular level is provided by the use of a binary matrix that enables phosphatidylcholines, sphingomyelins, phosphatidylserines, phosphatidylinositols and phosphoethanolamines to be detected via MALDI(±)-MS in both the positive and negative ion modes. An optimalMALDI-MS protocol for lipidomicmonitoring of a single intact embryo is therefore reportedwith potential applications in human and animal reproduction, cell development and stem cell research.
机译：在植入前的胚胎中，脂质在确定生存力，冷冻保存和植入特性方面起着关键作用，但是由于它们每个中存在少量分析物，它们的分析通常具有分析挑战性。基质辅助激光解吸/电离质谱（MALDI-MS）可以获取这些微生物的磷脂谱。该技术足够灵敏，能够分析单个完整的胚胎并在发育的早期阶段监测膜脂质组成的变化，用作研究繁殖生物学和生物技术的筛选方法。本文介绍了一种改进的，更全面的MALDI-MS脂质指纹识别方法，该方法可大大增加从单个胚胎获得的脂质信息。使用牛胚胎作为生物学模型，我们还在MALDI-MS分析之前测试了最佳的样品存储和处理条件。通过使用二元矩阵，可以在分子水平上提供改进的信息，该矩阵使磷脂酰胆碱，鞘磷脂，磷脂酰丝氨酸，磷脂酰肌醇和磷酸乙醇胺能够通过MALDI（±）-MS在正离子和负离子模式下进行检测。因此，报道了用于单个完整胚的脂质组学监测的最佳MALDI-MS方案，其在人和动物繁殖，细胞发育和干细胞研究中具有潜在的应用。

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