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首页> 外文期刊>Journal of mass spectrometry: JMS >Mass spectrometry imaging of surface lipids on intact Drosophila melanogaster flies
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Mass spectrometry imaging of surface lipids on intact Drosophila melanogaster flies

机译：完整果蝇果蝇表面脂质的质谱成像

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摘要

The spatial distribution of neutral lipids and semiochemicals on the surface of six-day-old separately reared naive Drosophila melanogaster flies has been visualized and studied using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and laser-assisted desorption/ionization (LDI)-TOF imaging (MSI). Metal targets were designed for twodimensional MSI of the surface of 3-D biological objects. Targets with either simple grooves or profiled holes designed to accurately accommodate the male and female bodies were fabricated. These grooves and especially holes ensured correct height fixation and spatial orientation of the flies on the targets after matrix application and sample drying. For LDI-TOF to be used, the flieswere arranged into holes and fixed to a plane of the target using fast-setting glue. InMALDI-TOFmode, the flieswere fixed as above and sprayed with a lithium 2,5-dihydroxybenzoate matrix using up to 100 airbrush spray cycles. The scanning electron microscopy images revealed that the deposits ofmatrix were homogenous and thematrix formedmostly into the clusters of crystals (40-80 μm) that were separated from each other by an uncovered cuticle surface (30-40 μm). The MSI using target with profiled holes provided superior results to the targetswith simple grooves, eliminating the ion suppression/mass deviation due to the 3-D shape of the flies. Attentionwas paid to neutral lipids and other compounds including the male anti-attractant 11-cis-vaccenyl acetate for which the expected distribution with high concentration on the tip of the male abdomen was confirmed. The red and blue mass shift (PlusMinus1 colour scale) was observed associated with mass deviation predominantly between ±0.2 and 0.3Da. We use in-house developed software formass recalibration, to eliminate themass deviation effects and help with the detection of low-intensity mass signals.
机译：使用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法和激光光谱法对六天大的单独饲养的初生果蝇果蝇表面上的中性脂质和化学信息素的空间分布进行了可视化和研究。辅助解吸/电离（LDI）-TOF成像（MSI）。金属靶被设计用于3-D生物物体表面的二维MSI。制作了带有简单凹槽或异型孔的目标，这些目标被设计用来精确地容纳公母体。这些凹槽，尤其是孔，确保在基质施加和样品干燥后，果蝇在目标上的正确高度固定和空间定向。对于要使用的LDI-TOF，将果蝇排成小孔，并使用快速固化胶将其固定在目标的平面上。在MALDI-TOF模式下，将果蝇如上固定，并使用多达100个喷枪喷雾周期用2,5-二羟基苯甲酸锂基质喷雾。扫描电子显微镜图像显示，基质的沉积是均匀的，并且基质主要形成晶体簇（40-80μm），所述晶体簇由未覆盖的表皮表面（30-40μm）彼此分开。 MSI使用带有异形孔的靶材，其结果优于具有简单凹槽的靶材，消除了由于果蝇的3D形状而引起的离子抑制/质量偏差。注意中性脂质和其他化合物，包括雄性抗吸引剂11-顺式-维菌烯基乙酸酯，已确认其在雄性腹部尖端具有高浓度的预期分布。观察到红色和蓝色质量偏移（PlusMinus1色标）与质量偏差主要在±0.2到0.3Da之间。我们使用内部开发的软件formass重新校准，以消除质量偏差效应，并帮助检测低强度质量信号。

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