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首页> 外文期刊>Journal of mass spectrometry: JMS >Quantitative confirmation of testosterone and epitestosterone in human urine by LC/Q-ToF mass spectrometry for doping control
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Quantitative confirmation of testosterone and epitestosterone in human urine by LC/Q-ToF mass spectrometry for doping control

机译：LC / Q-ToF质谱法定量检测人尿中睾丸激素和表睾酮的含量

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摘要

Testosterone (T) is the primary male sex hormone. In addition to the development of secondary sex characteristics, testosterone has anabolic effects including increases in muscle size and strength and increases in lean body mass, making it an attractive candidate to enhance athletic performance. In the case of exogenous administration of testosterone, the ratio of testosterone to its isomer, epitestosterone (E), is elevated. WADA has set a standard for TIE ratios of 4.0 as indicative of possible exogenous testosterone administration. Typically, a sample that screens for a TIE ratio above that threshold is then subjected to quantitative confirmation by GC/MS. This methodology, however, can limited due to sensitivity issues as well as a limited number of qualifying ions that can be used for unambiguous identification. We have developed a confirmation method which uses liquid/liquid extraction, followed by room temperature Girard P derivatization, and analysis using LC/MS-ToF. We observe a number of advantages over conventional GC/MS analysis. Analysis time is decreased. Sensitivity is increased, resulting in limits of detection of 2 and 0.5 ng/ml for testosterone and epitestosterone, respectively. The number of diagnostic qualifier ions is also increased allowing more confident identification of the analytes. Finally, while this method has been developed on a QToF instrument, it should be easily transferable to any tandem LC/MS/MS system.
机译：睾丸激素（T）是主要的男性性激素。除了发展第二性征外，睾丸激素还具有合成代谢作用，包括增加肌肉大小和强度以及增加瘦体重，使其成为提高运动表现的诱人候选人。在外源性施用睾丸激素的情况下，睾丸激素与其异构体表睾酮（E）的比率增加。 WADA为TIE比率设定了4.0的标准，以指示可能会进行外源性睾丸激素治疗。通常，筛选出高于该阈值的TIE比率的样品然后通过GC / MS进行定量确认。但是，由于灵敏度问题以及可用于明确识别的有限数量的合格离子，该方法可能会受到限制。我们已经开发出一种确认方法，该方法使用液/液萃取，然后进行室温Girard P衍生化，并使用LC / MS-ToF进行分析。与传统的GC / MS分析相比，我们发现了许多优势。分析时间减少了。灵敏度提高，导致睾丸激素和表睾酮的检出限分别为2和0.5 ng / ml。诊断定性离子的数量也增加了，从而可以更可靠地鉴定分析物。最后，尽管此方法已在QToF仪器上开发，但应易于转移至任何串联LC / MS / MS系统。

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