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首页> 外文期刊>Journal of mass spectrometry: JMS >Peptide fingerprinting of snake venoms by direct infusion nano-electrospray ionization mass spectrometry: potential use in venom identification and taxonomy
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Peptide fingerprinting of snake venoms by direct infusion nano-electrospray ionization mass spectrometry: potential use in venom identification and taxonomy

机译：直接注入纳米电喷雾电离质谱法对蛇毒进行肽指纹分析：在毒液鉴定和分类学中的潜在用途

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摘要

Fingerprinting by mass spectrometry has been increasingly used to study venom variations and for taxonomic analyses based on venom components. Most of these studies have concentrated on components heavier than 3 kDa, but Bothrops snake venoms contain many biologically active peptides, principally C-type natriuretic peptides and bradykinin-potentiating peptides (BPPs). In this work, we have examined the peptide profile of Bothrops venoms (B. alternatus, B. erythromelas, B. insularis, B. jararaca, B. jararacussu, B. leucurus and B. moojeni) using direct infusion nano-electrospray ionization mass spectrometry (nano-ESI-MS) subjecting the data further to principal components analysis (PCA) to assess whether the peptide distributions are reliable in distinguishing the venoms. ESI-MS of a low molar mass fraction obtained by ultrafiltration of each venom (5 kDa nominal cutoff filters) revealed that the venoms have a variety of peptides in common but that each venom also contains taxonomic marker peptides not shared with other venoms. One BPP peptide, QGGWPRPGPEIPP, was found to be common to the seven Bothrops species examined. This peptide may represent a specific marker for this genus since it was not found in the venom of the South American rattlesnake, Crotalus durissus terrificus. PCA on the ESI-MS data reveals a close relationship between B. jararaca, B. jararacussu and B. moojeni venoms, with B. leucurus and B. erythromelas being more distant from these three; B. alternatus and B. insularis were also located distant from these five species, as was C. d. terrificus. These results agree partially with established phylogenetic relationships among these species and suggest that ESI-MS peptide fingerprinting of snake venoms coupled with PCA is a useful tool for identifying venoms and for taxonomic analyses.
机译：质谱指纹已越来越多地用于研究毒液变化和基于毒液成分的分类分析。这些研究大多数集中在重于3 kDa的成分上，但是Bothrops蛇毒含有许多具有生物活性的肽，主要是C型利钠肽和缓激肽增强肽（BPP）。在这项工作中，我们已经使用直接输注纳米电喷雾电离质量检测了Bothrops毒液（B. alternatus，B。erythromelas，B。insularis，B。jararaca，B。jararacussu，B。leucurus和B. moojeni）的肽谱。光谱仪（nano-ESI-MS）对数据进行进一步的主成分分析（PCA），以评估肽段分布在区分毒液方面是否可靠。通过对每种毒液（5 kDa标称截止过滤器）进行超滤获得的低摩尔质量分数的ESI-MS显示，毒液共有多种肽，但每种毒液还包含与其他毒液不共享的分类标记肽。发现一种BPP肽QGGWPRPGPEIPP是所检查的七个Bothrops物种共有的。该肽可能代表了该属的特异性标记，因为在南美响尾蛇（Ctalalus durissus terrificus）的毒液中未发现该肽。在ESI-MS数据上的PCA揭示了jararacasu，jararacussu B.和moojeni毒液之间的密切关系，其中leucurus和erythromelas B.与这三者的距离更远。 B. alternatus和insularis B.也与这五个物种相距遥远。很棒这些结果与这些物种之间已建立的系统发育关系部分吻合，并表明蛇毒的ESI-MS肽指纹图谱与PCA结合是鉴定蛇毒和进行分类学分析的有用工具。

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