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首页> 外文期刊>Journal of mass spectrometry: JMS >Dereplication of Streptomyces sp. AMC 23 polyether ionophore antibiotics by accuratemass electrospray tandem mass spectrometry
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Dereplication of Streptomyces sp. AMC 23 polyether ionophore antibiotics by accuratemass electrospray tandem mass spectrometry

机译：链霉菌属的去复制。精确质量电喷雾串联质谱法测定AMC 23聚醚离子载体抗生素

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Actinomycetes, especially those belonging to the genus Streptomyces, are economically important from a biotechnological standpoint: they produce antibiotics, anticancer compounds and a variety of bioactive substances that are potentially applicable in the agrochemical and pharmaceutical industries. This paper combined accurate-mass electrospray tandem mass spectrometry in the full scan and product ion scan modes with compounds library data to identify the major compounds in the crude extract produced by Streptomyces sp. AMC 23; it also investigated how sodiated nonactin ([M + Na]~+) fragmented. Most product ions resulted from elimination of 184 mass units due to consecutive McLafferty-type rearrangements. The data allowed identification of four macrotetrolides homologous to nonactin (monactin, isodinactin, isotrinactin/trinactin and tetranactin) as well as three related linear dimer compounds (nonactyl nonactoate, nonactyl homononactoate and homononactyl homononactoate). The major product ions of the sodiated molecules of these compounds also originated from elimination of 184 and 198 mass units. UPLC-MS/MS in the neutral loss scan mode helped to identify these compounds on the basis of the elimination of 184 and 198 mass units. This method aided monitoring of the relative production of these compounds for 32 days and revealed that the biosynthetic process began with increased production of linear dimers as compared with macrotetrolides. These data could facilitate dereplication and identification of these compounds in other microbial crude extracts.
机译：从生物技术的角度看，放线菌，特别是属于链霉菌属的那些，在经济上很重要：它们产生抗生素，抗癌化合物和多种可能在农业化学和制药工业中应用的生物活性物质。本文将全质谱和产物离子扫描模式下的精确质量电喷雾串联质谱与化合物库数据结合起来，以鉴定链霉菌属菌种粗提物中的主要化合物。 AMC 23;它还研究了如何使游离的非肌动蛋白（[M + Na]〜+）片段化。大多数产物离子是由于连续McLafferty型重排消除了184质量单位而产生的。数据可鉴定出与非肌动蛋白同源的四个大四环内酯（monactin，异dinactin，异素肌动蛋白/三肌动蛋白和四联肌动蛋白）以及三个相关的线性二聚体化合物（壬酸壬酸壬酸酯，壬酸壬壬酸壬酸酯和高壬酸壬壬酸壬酸酯）。这些化合物的固态分子的主要产物离子也源自消除184和198质量单位。中性损失扫描模式下的UPLC-MS / MS在消除184和198质量单位的基础上帮助鉴定了这些化合物。该方法有助于监测这些化合物的相对生成量，为期32天，结果表明，与大四环内酯相比，生物合成过程始于线性二聚体的生成增加。这些数据可以促进其他微生物粗提物中这些化合物的重复复制和鉴定。

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