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首页> 外文期刊>Journal of mass spectrometry: JMS >Peptides derivatized with bicyclic quaternary ammonium ionization tags. Sequencing via tandem mass spectrometry
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Peptides derivatized with bicyclic quaternary ammonium ionization tags. Sequencing via tandem mass spectrometry

机译：用双环季铵离子化标签衍生的肽。通过串联质谱法测序

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摘要

Improving the sensitivity of detection and fragmentation of peptides to provide reliable sequencing of peptides is an important goal of mass spectrometric analysis. Peptides derivatized by bicyclic quaternary ammonium ionization tags: 1-azabicyclo[2.2.2]octane (ABCO) or 1,4-diazabicyclo[2.2.2]octane (DABCO), are characterized by an increased detection sensitivity in electrospray ionization mass spectrometry (ESI-MS) and longer retention times on the reverse-phase (RP) chromatography columns. The improvement of the detection limit was observed even for peptides dissolved in 10mM NaCl. Collision-induced dissociation tandem mass spectrometry of quaternary ammonium salts derivatives of peptides showed dominant a- and b-type ions, allowing facile sequencing of peptides. The bicyclic ionization tags are stable in collision-induced dissociation experiments, and the resulted fragmentation pattern is not significantly influenced by either acidic or basic amino acid residues in the peptide sequence. Obtained results indicate the general usefulness of the bicyclic quaternary ammonium ionization tags for ESI-MS/MS sequencing of peptides. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译：提高肽的检测和片段化的灵敏度以提供可靠的肽测序是质谱分析的重要目标。由双环季铵离子化标签衍生的肽：1-氮杂双环[2.2.2]辛烷（ABCO）或1,4-二氮杂双环[2.2.2]辛烷（DABCO），其特征在于电喷雾电离质谱的检测灵敏度提高（ ESI-MS）和更长的反相（RP）色谱柱保留时间。即使对于溶解在10mM NaCl中的肽，也可以观察到检测限的提高。肽的季铵盐衍生物的碰撞诱导解离串联质谱分析显示主要的a型和b型离子，可轻松进行肽测序。双环电离标签在碰撞诱导的解离实验中是稳定的，并且所得的片段化模式不受肽序列中酸性或碱性氨基酸残基的影响。获得的结果表明双环季铵离子化标签对于ESI-MS / MS肽测序的一般用途。版权所有（c）2014 John Wiley＆Sons，Ltd.

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