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首页> 外文期刊>Journal of mass spectrometry: JMS >Mass spectrometry imaging: a new vision in differentiating Schistosoma mansoni strains
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Mass spectrometry imaging: a new vision in differentiating Schistosoma mansoni strains

机译：质谱成像：区分曼氏血吸虫菌株的新视野

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摘要

Schistosomiasis is a neglected disease with large geographic distribution worldwide. Among the several different species of this parasite, S. mansoni is the most common and relevant one; its pathogenesis is also known to vary according to the worms' strain. High parasitical virulence is directly related to granulomatous reactions in the host's liver, and might be influenced by one or more molecules involved in a specific metabolic pathway. Therefore, better understanding the metabolic profile of these organisms is necessary, especially for an increased potential of unraveling strain virulence mechanisms and resistance to existing treatments. In this report, MALDI-MSI and the metabolomic platform were employed to characterize and differentiate two Brazilian S. mansoni strains: males and females from Belo Horizonte (BH) and from Sergipe (SE). By performing direct analysis, it is possible to distinguish the sex of adult worms, as well as identify the spatial distribution of chemical markers. Phospholipids, diacylglycerols and triacylglycerols were located in specific structures of the worms' bodies, such as tegument, suckers, reproductive and digestive systems. Lipid profiles were found to be different both between strains and males or females, giving specific metabolic fingerprints for each group. This indicates that biochemical characterization of adult S. mansoni may help narrowing-down the investigation of new therapeutic targets according to worm composition, molecule distribution and, therefore, aggressiveness of disease.
机译：血吸虫病是一种被忽视的疾病，在全球范围内分布广泛。在这种寄生虫的几种不同物种中，曼氏葡萄球菌是最常见和最相关的一种。还已知其发病机理根据蠕虫的品系而变化。较高的寄生毒力与宿主肝脏中的肉芽肿反应直接相关，并且可能受特定代谢途径中涉及的一种或多种分子的影响。因此，有必要更好地了解这些生物的代谢特征，特别是对于提高菌株毒力机理和对现有治疗方法的抵抗力的潜力。在本报告中，MALDI-MSI和代谢组学平台用于表征和区分两种巴西曼氏沙门氏菌菌株：来自贝洛奥里藏特（BH）和塞尔希培州（SE）的雄性和雌性。通过直接分析，可以区分成虫的性别，并确定化学标记的空间分布。磷脂，二酰基甘油和三酰基甘油位于蠕虫身体的特定结构中，例如外皮，吸盘，生殖和消化系统。发现品系和雄性或雌性之间的脂质谱均不同，从而为每组提供了特定的代谢指纹。这表明成年曼氏葡萄球菌的生化特性可能有助于根据蠕虫的成分，分子分布以及因此对疾病的侵袭性，缩小对新治疗靶标的研究范围。

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