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首页> 外文期刊>Journal of mass spectrometry: JMS >Analysis of the phenolic composition of fungusresistant grape varieties cultivated in Italy and Germany using UHPLC-MS/MS
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Analysis of the phenolic composition of fungusresistant grape varieties cultivated in Italy and Germany using UHPLC-MS/MS

机译：使用UHPLC-MS / MS分析意大利和德国种植的抗真菌葡萄品种的酚类成分

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摘要

The phenolic composition of grape berries grown in Italy (Cabernet Cortis, Johanniter, Solaris, Phoenix, and Regent) and in Germany (Regent and Phoenix) was analyzed using two complementary LC-MS/MS (Liquid Chromatography - Tandem Quadrupole Mass Spectrometry) methods. These five cultivars belong to fungus-resistant grape varieties (PIWI) and little is known about their phenolic profile, although phenolics such as phytoalexins have been recognized, playing a key role in the resistance mechanism. A triple quadruple mass spectrometer detector was used for both identification and quantification, and analytical tools from untargeted metabolomics were applied to check and control the quality of the results. Specifically, biological, technical, and instrumental replications were included in order to study the variability at different levels. The results allowed tuning of the sampling protocol and provided a rich phenolic metabolite profile for the aforementioned PIWI varieties, as compared with Vitis vinifera cultivars, especially in the class of stilbenes.
机译：使用两种互补的LC-MS / MS（液相色谱-串联四极杆质谱）方法分析了在意大利（Cabernet Cortis，Johanniter，Solaris，Phoenix和Regent）和在德国（Regent和Phoenix）种植的葡萄浆果的酚类成分。 。这五个品种均属于抗真菌葡萄品种（PIWI），尽管对酚类物质（如植物抗毒素）的识别已在抗性机制中发挥了关键作用，但对它们的酚类特性知之甚少。使用三重四重质谱仪检测器进行鉴定和定量，并使用来自非目标代谢组学的分析工具来检查和控制结果的质量。具体来说，包括生物学，技术和仪器复制，以研究不同水平的变异性。与葡萄，特别是在斯蒂芬类中相比，该结果允许调整采样方案并为上述PIWI品种提供丰富的酚代谢物谱。

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