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首页> 外文期刊>Journal of mass spectrometry: JMS >Evaluation of analytical performance and reliability of direct nanoLC-nanoESI-high resolution mass spectrometry for profiling the (xeno)metabolome
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Evaluation of analytical performance and reliability of direct nanoLC-nanoESI-high resolution mass spectrometry for profiling the (xeno)metabolome

机译：评估直接纳米LC-nanoESI-高分辨率质谱分析（异种）代谢组的分析性能和可靠性

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摘要

Mass spectrometry (MS) profiling techniques are used for analysing metabolites and xenobiotics in biofluids; however, detection of low abundance compounds using conventional MS techniques is poor. To counter this, nanoflow ultra-high-pressure liquid chromatography-nanoelectrospray ionization-time-of-flight MS (nUHPLC-nESI-TOFMS), which has been used primarily for proteomics, offers an innovative prospect for profiling small molecules. Compared to conventional UHPLC-ESI-TOFMS, nUHPLC-nESI-TOFMS enhanced detection limits of a variety of (xeno)metabolites by between 2 and 2000-fold. In addition, this study demonstrates for the first time excellent repeatability and reproducibility for analysis of urine and plasma samples using nUHPLC-nESI-TOFMS, supporting implementation of this platform as a novel approach for high-throughput (xeno)metabolomics. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译：质谱（MS）分析技术用于分析生物流体中的代谢物和异生物素。但是，使用常规质谱技术检测低丰度化合物的效果较差。为了解决这个问题，主要用于蛋白质组学的纳流超高压液相色谱-纳米电喷雾电离飞行时间质谱仪（nUHPLC-nESI-TOFMS）为小分子分析提供了创新的前景。与常规UHPLC-ESI-TOFMS相比，nUHPLC-nESI-TOFMS将多种（异种）代谢物的检测限提高了2到2000倍。此外，本研究首次展示了使用nUHPLC-nESI-TOFMS分析尿液和血浆样品的出色重复性和重现性，支持该平台作为高通量（异种）代谢组学的一种新方法的实施。版权所有（c）2014 John Wiley＆Sons，Ltd.

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